胆管癌中Livin蛋白和血管内皮生长因子的表达及与Caspase-3的相关性研究

目的：探讨凋亡抑制因子Livin蛋白和血管内皮生长因子（vascular endothelial growth factor,VEGF）在胆管癌组织中的表达及与Caspase-3的相关性.方法：采用免疫组化SP法检测80例胆管癌及30例正常胆管组织（取自其他良性疾病行胆管切除者）中Livin蛋白、VEGF及Caspase-3的表达.结果：胆管癌组织中Livin蛋白和VEGF的表达明显高于正常胆管组织（P=0.00）,Caspase-3的表达低于正常胆管组织（P=0.04）;在胆管癌组织中Livin蛋白、VEGF的表达具有正相关性（r=0.532,P=0.00）,Livin蛋白、VEGF的表达均与Caspase-3的表达呈负相关关系（r=-0.668,P=0.00;r=-0.371,P=0.01）.结论：Livin蛋白、VEGF在胆管癌组织中均高表达及两者呈正相关性,表明两者在胆管癌的发生发展中可能起重要作用并且可能发挥协同作用,二者的表达均与Caspase-3的表达呈负相关关系,提示二者均可能是通过抑制凋亡来参与胆管癌的发生发展.

Expression of Livin protein and VEGF and their relationship with Caspase-3 in cholangiocarcinoma

Objective：To study the expression of Livin protein and vascular endothelial growth factor（VEGF） in cholangiocarcinoma tissue and their relationship with Caspase -3. Methods： The expression of Livin protein, VEGF and Caspase - 3 were detected by immunohistochemistry SP in 80 cases of cholangiocarcinoma and 30 cases of normal bile duct tissue（ from patients with other benign diseases who had cut off the bile duct）. Results： The expressions of Livin protein and VEGF in cholangiocarcinoma tissue were obviously higher than in normal bile duct tissue（P = O. 00） ,but the expression of Caspase -3 was obviously lower than in normal bile duct tissue（ P =0.04） ;In bile duct tissue,the expressions of Livin protein and VEGF were positive correlation （r = 0. 532,P = 0. 00） , the expression of Livin protein and VEGF were negative correlation with Caspase - 3 （ r = - 0. 668, P = 0. 00 ; r = - 0.371, P = 0.01 ）. Conclusion： Livin protein and VEGF were high expression in cholangiocarcinoma tissue with positive correlation, in- dicating that they may play a significant role in the growth and development of cholangiocarcinoma with synergistic ac- tion. Because they both had negative correlation with Caspase -3,indicating that they may participate in the growth and development of cholangiocarcinoma by inhibiting apoptosis.