HPLC法测定鸡矢藤药材中环烯醚萜苷含量

目的:采用高效液相色谱和二极管阵列检测器法(HPLC-DAD)同时测定鸡矢藤药材中3种主要环烯醚萜苷类成分的含量,研究不同产地鸡矢藤药材中环烯醚萜苷的含量.方法:色谱分析采用Shim-pack VP-ODS C18分析柱,0.1%的甲酸(A)和乙腈(B)为流动相进行梯度洗脱,检测波长:240nm.结果:3个环烯醚萜苷[鸡矢藤苷酸(1)、鸡矢藤苷(2)、鸡矢藤苷酸甲酯(3)]在标准曲线内均有较好的线性(r2>0.9999),且该方法具有良好的精密度,灵敏度,重复性和准确度;应用所建立的方法同时测定了8批鸡矢藤药材中三个环烯醚萜苷的含量.结论:本文首次报道建立HPLC-DAD方法同时测定鸡矢藤中主要环烯醚萜苷类成分含量,该方法简单、快速、可靠,适用于鸡矢藤药材及其制剂的质量控制.

HPLC Analysis of Iridoid Glycosides from the Stem of Paederia scandens

T Objective: To establish a quantitative analysis method with HPLC-DAD on simultaneous determination of iridoid glycosides in the stem of Paederia scandens from different place of production for quality control. Methods: A rapid and accurate HPLC-DAD method was developed and validated for the quantitative determination of three iridoid glycosides: paederosidic acid (1), paederoside (2) and methyl paederosidate (3). The subsequent HPLC separation and quantification was achieved in 45minutes using a Shim -pack VP -ODS C18 column and at 240nm The mobile phase comprised solvent A (0.1% formic acid aqueous, V/V) and solvent B (acetonitrile) and a gradient elution mode was applied. This established method was therefore applied to determining the amounts of iridoid glycosides in eight samples collected from different regions in China. Results: The linear regression analytical data for the calibration plots showed a good linear relationship with r2>0.9999 within the tested ranges. The average recoveries were from 98.47% to 104.85% indicating good accuracy. It was successfully applied to quantifying the three iridoid glycosides in different samples. Conclusion: It is the first time to establish a quantitative method to investigate the contents of iridoid glycosides in Paederia scandens from different places of production, and this method can be applied to the quality control of raw material Paederia scandens.