血管内皮生长因子、肝细胞生长因子在虹膜新生血管大鼠房水中的含量变化

目的：探讨血管内皮生长因子（vascular endothelial growth factor，VEGF）、肝细胞生长因子（hepatocyte growth factor，HGF）在虹膜新生血管（neovascularization of the iris，NVI）大鼠模型房水中的含量变化。方法将清洁级健康10周龄Wistar大鼠随机分为4组，每组20只（40眼），乙醚吸入法全身麻醉大鼠，实验1组：经尾部静脉注射孟加拉玫瑰红光敏剂；实验2组：尾部静脉注射孟加拉玫瑰红光敏剂＋氪激光光凝Wistar大鼠全部视网膜分支静脉；实验3组：氪激光光凝Wistar大鼠全部视网膜分支静脉；实验4组：空白对照组（未经任何处理）。利用虹膜荧光造影（iris fluorescence angiography，IFA）对各实验组大鼠进行鉴定，对NVI动物模型复制成功的大鼠采用Miller方法进行分级，对大鼠NVI形成前1天及模型复制后3、7、10、14、21、30 d分别抽取房水，采用酶联免疫吸附实验（enzyme-linked immunosorbent assay，ELISA）检测房水中VEGF、HGF的含量。采用SPSS 13.0统计软件分析实验组及对照组之间不同时间组房水中VEGF、HGF 含量变化，并分析两种因子的相关性。结果单纯应用孟加拉玫瑰红尾部静脉注射和单纯激光的方法不能使大鼠产生视网膜缺血，用尾部静脉注射孟加拉玫瑰红光敏剂＋氪激光光凝法可诱导大鼠视网膜静脉阻塞，成功制作NVI动物模型。 NVI模型复制前1 d房水中VEGF和HGF的浓度分别为（15.58±5.85）pg/ml和（22.84±6.75）pg/ml，复制后3、7、10、14、21、30 d二者浓度分别为（17.30±4.80）、（31.83±9.90）、（36.89±11.73）、（57.34±18.55）、（112.03±46.41）、（106.93±52.89）pg/ml和（24.58±7.18）、（36.47±11.28）、（46.27±13.11）、（59.25±15.47）、（90.71±29.91）、（102.08±34.82）pg/ml；VEGF和HGF与NVI形成呈正相关，但二者浓度无相关性。结论大鼠虹膜新生血管的形成与其房水中VEGF、HGF含量成正相关，二者在虹膜新生血管形成中可能扮演重要角色。

The content variation of VEGF and HGF in aqueous humor in rats in iris neovascularization

Objective To discuss the content variation of VEGF and HGF in aqueous humor in rats in iris neovascularization. Methods The eighty clean health level 10 weeks of Wistar rats were randomly divided into 4 groups and ether inhalation anesthesia. Groups 1： by tail intravenous injection of 3,4,5,6-Tetrachlorofluorescein;groups 2： tail intravenous injection of 3,4,5,6-Tetrachlorofluorescein＋krypton laser photocoagulation in Wistar rats all branch retinal vein;group 3：krypton laser photocoagulation in Wistar rats all branch retinal vein;group 4：control group（without any treatment）. The NVI success rat model identification was used iris fluorescence angiography technology. Then, the NVI success rat models were classed by Miller methods. The rat aqueous fluid was abstracted on 1 day before and after 3, 7, 10, 14, 21, 30 days model respectively. The concentration of VEGF and HGF of aqueous fluid were detected by enzyme-linked immunosorbent assay. The differences between the experimental data, the correlation analysis were completed by SPSS 13.0 software. Results Single application of Bangladesh rosy tail vein injection and single laser method didn’t cause retinal ischemia in rats, but the tail vein injection of Bangladesh rosy photosensitizer＋krypton laser photocoagulation method could induced retinal vein occlusion in rats. It successfully produced NVI animal model. NVI model replication before 1 day of VEGF and HGF concentrations were （15.58±5.85） pg/ml and （22.84±6.75） pg/ml, respectively. And the VEGF and HGF concentrations after 7, 10, 3, 14, 21 30 days were （17.30±4.80）, （31.83±9.90）, （36.89±11.73）, （57.34±18.55）, （112.03±46.41）, （106.93±52.89） pg/ml and （24.58±7.18）, （36.47±11.28）, （46.27±13.11）, （59.25±15.47）, （90.71±29.91）, （102.08±34.82） pg/ml, respectively. VEGF and HGF and NVI formation were positively correlated, but aqueous HGF concentrations were unrelated to those of VEGF. Conclusion It presented positive correlation between the formation of neovascularization of the Iris in rats and VEGF, HGF content in aqueous humor. The VEGF and HGF played an important role in the iris neovascularization.