肺结核患者单核细胞趋化因子蛋白-1基因多态性的检测

目的探讨我国北方地区汉族人群单核细胞趋化因子蛋白（MCP-1）-2518位基因多态性与肺结核的相关性。方法应用聚合酶链反应-序列特异性引物（PCR—SSP）和聚合酶链反应．限制性片段长度多态性（PCR-RFLP）的方法检测我国北方地区汉族136例肺结核患者和152例正常人对照的MCP-1基因．2518位基因多态性。结果肺结核患者中GG基因型的频率明显高于对照组（P〈0．05，OR=1．96）：AA和AG基因型的频率在两组间无统计学差异（P〉O．05）。肺结核患者中等位基因G的频率明显高于对照组（P〈O．05，OR=1．536）。MCP-1基因多态性在初治与复治、耐药与无耐药患者中的频率分布无显著差异（P〉0．05）。结论中国北方地区汉族人群MCP-1基因-2518位点多态性与肺结核具有相关性，GG基因型可能是肺结核的易感因素。

Detection of genetic polymorphism of MCP-1 in pulmonary tuberculosis in Han nationality of North China

Objective To study the relationship between monocyte chemoattraetant protein l（MCP-I）-2518A/ G polymorphism and pulmonary tuberculosis（PTB） in Hart nationality of North China. Methods 136 unrelated consecutive patients with PTB and 152 healthy individuals were studied. The polymorphisms of MCP-1-2518A/G were detected with polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）. Results There was a significant increase in the frequency of the GG genotype in PTB patients compared to controls（P〈0.05, OR=1.96）. The distribution of AA and AG genotypes of MCP-1-2518 was not significantly different in PTB patients compared with controls（P〉0.05）. There was a significant increase in the frequency of the G allele in PTB patients compared to controls（P〈0.05, OR= 1.536）. The gene polymorphism of MCP-1-2518 was not significantly different in PTB patients of initial treatment, retreatment, drug sensitive and drug resistant（P〉0.05）. Conclusion This preliminary study showed that MCP-1-2518A/G polymorphism was associated with PTB. MCP-1 GG genotype may be the susceptible factor of PTB.