细胞因子诱导的杀伤细胞对卵巢癌细胞株SKOV-3的杀伤效应

目的探讨多种细胞因子诱导的杀伤细胞（CIK）对卵巢癌细胞株SKOV-3的杀伤效应。方法用健康人外周血单核细胞（PBMC）在体外用多种细胞因子诱导成CIK细胞，流式细胞术分析其表型特征，将培养14d的CIK细胞和对数生长期的SKOV-3细胞按不同效靶比共培养，MTT法检测CIK细胞对SKOV-3细胞的杀伤效应；将CIK细胞培养液上清作用于对数生长期的SKOV-3细胞，于培养24及48h用流式细胞仪检测卵巢癌细胞的凋亡情况。结果CIK细胞体外培养14d，CD3^＋CD56^＋双阳性细胞数量达（31．6±5．5）％。CIK对SKOV-3的杀伤效应在效靶比为5：1时，24h及48h抑制率分别为（16．52±2．52）％和（24．20±5．59）％，当效靶比为40：1时，其24h及48h抑制率分别为（53．98±5．02）％和（74．74±6．87）％。CIK细胞对SKOV-3细胞的抑制率随着效靶比的提高及作用时间的延长而提高。SKOV-3与CIK细胞培养液上清共培养24h及48h后凋亡率分别为（12．30±1．47）％和（27．13±2．03）％。结论CIK细胞可通过诱导卵巢癌SKOV-3细胞凋亡发挥较强的杀伤作用。

The anti-tumor effects of CIK against ovarian cancer cell lines SKOV-3 in vitro

Objective To investigate the anti-tumor effects of cytokine-induced kill cells（CIK） agaimt ovarian cancer cell lines SKOV-3 in vitro. Methods Peripheral blood mononuclear Cells（PBMC） of healthy human were stimulated by different cytokines, and were induced into CIK cells.The phenotype of CIK cells were analyzed by a flow cytometer. CIK cells cultured for 14 days and logarithmic phase SKOV-3 lines were taken as effect cells and target cells respectively. Effect cells were co-cultured with target cells at different ratio.The antitumor effect of CIK cells against SKOV-3 lines was measured by MTT. SKOV-3 cells were cultured with the CIK cells culturing supernatant for 24 and 48 hours and the SKOV-3 cells apoptosis was analyzed by a flow cytometer. Results The CD3^＋CD56^＋ double positive cells in the ratio was up to （31.6±5.5）% after 14 days culture. The killing activity increased with the cultured time prolong and the effector-target ratio increase. The maximum killing efficiency was about （74.74±6.87）% after co-culturing 48 hours with the effector-target ratio 40 ： 1. The CIK cells supematant can induce apoptosis of SKOV-3 cells.The apoptosis rates were （12.30±1.47） % and （27.13±2.03）% respectively after co-culturing 24 and 48 hours. Conclusion CIK cells have strong anti-cancer activity against SKOV-3 cells in vitro by inducing apoptosis of SKOV-3 cells.