有机酸对人血管内皮细胞的保护作用

目的：探讨氯原酸（CHA）、抗坏血酸（AA）、柠檬酸（CA）和苹果酸（MA）4种有机酸对人血管内皮细胞的保护作用。方法：通过人脐静脉内皮细胞培养，从形态、生长状况和乳酸脱氢酶（LDH）释放方面，观察预先4h、与oxLDL同时加入、加oxLDL3h后加入有机酸3种情况，以及3个不同剂量（10、20、40mg/L)的有机酸＋oxLDL(100mg/L)对内皮细胞的作用。结果:oxLDL对内皮细胞具有明显的损伤作用，细胞生存率低于正常对照组，LDH释放率高于正常组。预先和同时加入CAH、AA、CA、和MA各组对oxLDL引起的内皮细胞损伤具有不同程度的保护作用，而后加入有机酸各组对oxLDL引起的内皮细胞损伤没有影响。3个剂量的CHA和AA＋oxLDL各组，以及CA和MA＋oxLDL的高剂量组的细胞生存率均高于oxLDL组，LDH释放率均低于ocxLDL组，呈明显的剂量效应关系。结论、氯原酸、抗坏血酸、柠檬酸和苹果酸4种有机酸对oxLDL诱导的内皮细胞损伤具有不同程度的预防性保护作用，其中以氯原酸和抗坏血酸的作用最佳，柠檬酸次之，苹果酸最终。

Protective effects of organic acids on human vascular endothelial cells

Objectives In order to examine if organic acids (OA),a kind of natural food components,will prevent from atherosclerosis formation,the effects of four OA,namely chlorogenic acid (CHA),ascorbic acid (AA),citric acid (CA),and malic acid (MA) on human vascular endothelial cells were studied. Methods Primary human umbilical vein endothelial cells (EC) were cultured,and the changes of cell morphology,cell growth,lactate dehydrogenase (LDH) released from cells were measured when EC was incubated with oxidized low density lipoprotein (oxLDL) in the presence or not of four OA added at three different conditions,i e. before 4 hours oxLDL added,at the same time as oxLDL added,and after 3 hours oxLDL added,as well as at the indicated doses (10,20 or 40 mg/L). Results The EC survival rate of the oxLDL control group was lower ( P <0 01) and LDH release rate was higher ( P <0 01) than those of normal control group CHA,AA,CA and MA protected EC from damage caused by oxLDL when they were added before and/or at the same time of oxLDL,but the same beneficial effects of these four OA added after oxLDL were not observed The EC survival rates of CHA oxLDL and AA oxLDL groups in three doses were higher ( P <0 05) and the LDH release rates were lower ( P <0 05) in a dose effect manner when compared with those of oxLDL control group,respectively Similar results were found in the CA oxLDL and MA oxLDL of the high dose groups. Conclusion Four tested OA could effectively prevent EC from damage induced by oxLDL CHA and AA had the strongest effects,the next was CA,followed by MA