山茶属植物的抗骨质疏松作用

目的:研究山茶属植物在等生药剂量下对大鼠实验性骨质疏松的拮抗作用,并对其活性进行比较。方法:W istar大鼠随机分为正常对照组、模型对照组、山茶和油茶不同提取物组及龙牡壮骨颗粒5 g/kg组。通过维甲酸造成大鼠实验性骨质疏松模型,再观察山茶和油茶不同提取物对骨质疏松大鼠股骨重量/长度(G/L)值、骨密度、无机物及骨钙含量、形态学改变和血清钙、抗酒石酸酸性磷酸酶(s-TRAP)、碱性磷酸酶(ALP)水平的影响,并在等生药剂量下对其作用强度进行比较。结果:各给药组同模型组比较:山茶子醇提物0.51 g/kg对G/L比值、骨密度有明显的提高作用;可明显提高血清钙和碱性磷酸酶水平,降低抗酒石酸酸性磷酸酶水平;形态学观察可见成骨细胞明显增多,破骨细胞明显减少;骨小梁明显向正常水平转化。山茶子醇提物抗维甲酸大鼠实验性骨质疏松作用最强,强于油茶叶醇提物及水提物,并强于阳性药。其余提取物抗骨质疏松作用不明显。各药物抗骨质疏松作用强弱顺序为:山茶子醇提物>油茶叶醇提物>油茶叶水提物>油茶子水提物>阳性药>山茶子水提物。结论:山茶属植物具有不同程度的抗骨质疏松作用,这为本属植物的进一步研究开发提供了重要的理论依据。

Study on the Theraputic Effect of Plants of Camellia Genus on Osteoporosis

Objective: To observe anti-osteoporotic effect of Plants of Camelia genus induced by retinoic acid in rats,in adqulis crude drug dosage,and to compare activities of them.Methods: Extracts of Camellia japonica and Camellia oleifera were given to rats with osteoporosis induced by retinoic acid,some indexes of rats were measured and compared with those of modle group,control group and positive control group,including weight/length(G/L),bone density,earth and calcium content of bone,morphology change and serum calcium,tartrate-resistant acid phosphatase and alkaline phosphatase.We also compared effective intensity between different groups in adqulis crude drug dosage.Results: Ethanol extracts of seed from Camellia japonica 0.51 g/kg could markedly enhance weight /length(G/L),bone density of femur,serum calcium and alkaline phosphatase level,with the decreasing of anti-tartaric acid tartrate-resistant acid phosphatase level.Meanwhile,they were accompanied by a significant increase of morphologic observed sclerotomal cell and by a significant decrease of osteoclast.Moreover,it was observed greatly that bone trabecula transformateed to normal morphous.The results of this study indicated that effects of ethanol extracts of seed from Camellia japonica On anti-osteoporosis with retinoic acid were the strongest.Ethanol extracts of seed from Camellia japonica,ethanol extracts of leaves from Camellia Oleifera,and aqueous extracts of leaves from Camellia Oleifera were stronger than positive control drug.The other extracts didn't show obvious anti-osteoporotic effects.Eventually the strength order of each group on anti-osteoporosis was as following: ethanol extracts of seed from Camellia japonica > ethanol extracts of leaves from Camellia Oleifera > aqueous extracts of leaves from Camellia Oleifera >aqueous extracts of seed from Camellia Oleifera > positive control drug >aqueous extracts of seed from Camellia Japonica.Conclusion:Plants of Camellia genus have different degree anti-osteoporosis effect,which can offer significant theory basis for progressive investigation and exploitation of them.