次声作用对小鼠骨髓多染性红细胞微核的影响

目的　研究次声对小鼠骨髓细胞染色体的损伤效应 .方法　 BAL B/ c小鼠经 16 Hz,12 0 d B,2 h· d- 1 的次声分别作用 1,3,7,10 d后取股骨 .通过 PCE- MNT法和骨髓细胞染色体制备 ,检测并观察多染性红细胞微核率和分裂相染色体的变化 .结果　与对照组 (雄鼠 :0 .2 6‰ ,0 .35‰ ,0 .41‰ ,0 .40‰ ;雌鼠 :0 .2 6‰ ,0 .32‰ ,0 .38‰ ,0 .33‰ )相比 ,次声组 (雄鼠 :0 .45‰ ,0 .6 0‰ ,0 .81‰ ,0 .85‰ ;雌鼠 :0 .42‰ ,0 .6 0‰ ,0 .92‰ ,0 .72‰ )的平均微核率增加显著(P<0 .0 5 ) .次声组平均微核率的增幅随作用天数的延长而显著增加 (P<0 .0 5 ) ;雌雄小鼠间的平均微核率无显著差异(P>0 .0 5 ) .骨髓细胞分裂相检测明显可见有染色体结构畸变现象 .结论　次声作用对小鼠骨髓多染性红细胞遗传损伤显著 .在本实验条件下 ,次声作用存在时间累积效应 ,雌雄小鼠对其反应敏感性相同 .

Effects of infrasound exposure on micronucleus of bone marrow polychromatic erythroblasts in mice

AIM To study the damage of chromosome in bone marrow cell of mice exposed to infrasound. METHODS BALB/c mice were exposed to infra sound of 16 Hz, 120 dB, 2 hours per day. Then femora were obtained after 1, 3, 7, 10 days respectively. The rates of micronucleus (MN) in polychromatophilic erythroblast (PCE) were detected by PCE-MNT and the chromosomes in division phase were observed. RESULTS Mean rate of MN in infrasound group increased markedly compared with that of the control group (P<0.05). The mean rates of MN in infrasound group increased significantly (P<0.05) with the increase of infrasound exposure. No significant difference of the mean rates of MN was detected between male mice and female mice (P>0.05). Visible structural aberration was observed distinctly in division phase of bone marrow cells. CONCLUSION Infrasound exposure causes significant genetic damage to PCE of mice, male or female alike. The more days of infrasound exposure, the more serious the damage will be.