| 表没食子儿茶素没食子酸酯对裸鼠肝癌移植瘤细胞凋亡作用的研究 |
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| 引用本文： | 欧冰凝,林晓贞,梁钢,孙悦文. 表没食子儿茶素没食子酸酯对裸鼠肝癌移植瘤细胞凋亡作用的研究[J]. 时珍国医国药, 2012, 23(8): 1854-1855 |
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| 作者姓名： | 欧冰凝 林晓贞 梁钢 孙悦文 |
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| 作者单位： | 广西医科大学药学院,广西南宁,530021 |
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| 基金项目： | 国家自然科学基金(No.30760310);广西壮族自治区自然科学基金(No.2010GXNSFD013043);广西医学科学实验中心开放基金(No.KFJJ2011-38) |
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| 摘 要： | 目的 了解表没食子儿茶素没食子酸酯(EGCG)对裸鼠肝癌移植瘤细胞凋亡的影响.方法 建立BEL-7404/ADR细胞裸鼠移植瘤模型,分为control组、ADM组、EGCG组、ADM联合低剂量EGCG组(AE low组)、ADM联合中剂量EGCG组(AE mid组)和ADM联合高剂量EGCG组(AE high组),给药20 d,处死裸鼠去瘤块.用免疫组织化学法(TUNEL法)检测瘤组织内原位细胞凋亡和流式细胞仪(FACS)检测肿瘤组织细胞周期.结果 ADM与低、中、高剂量的EGCG合用,其细胞凋亡率分别为16.7％,20.3％和25.9％,高于单用ADM组的9.3％(P＜0.01),使肿瘤细胞增殖阻滞在S/G2期.结论 EGCG可协同增强ADM促肝癌MDR细胞BEL-7404/ADR的凋亡,使肿瘤细胞增殖周期阻滞在S/G2期.
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| 关 键 词： | EGCG 肝癌细胞 凋亡 体内 |
The-Epigallocatechin Gallate and Adriamycin Apoptosis of BEL-7404/ADR in vivo |
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| OU Bing-ning , LIN Xiao-zhen , LIANG Gang , SUN Yue-wen. The-Epigallocatechin Gallate and Adriamycin Apoptosis of BEL-7404/ADR in vivo[J]. Lishizhen Medicine and Materia Medica Research, 2012, 23(8): 1854-1855 |
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| Authors: | OU Bing-ning LIN Xiao-zhen LIANG Gang SUN Yue-wen |
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| Affiliation: | (Pharmacological College of Guangxi Medical University,Nanning 530021,China) |
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| Abstract: | Objective To study on the-epigallocatechin gallate and adriamycin(ADM) apoptosis of BEL-7404/ADR in vivo.Methods The model of xenografted tumor in nude mice was established with BEL-7404/Adr.The experiment was divided into control group,ADM group,EGCG group and low,middle,high AE groups and received the desired drugs and dosage for 20 days.The apoptosis of BEL-7404/ADR cells was detected by TUNEL method and the cell cycle was tested by FACS.Results The rates of cell apoptosis was 16.7% low AE group in 20.3% middle AE group and 25.9% in high AE group,and were all in higher than that of ADM group(9.3%)(P<0.01).EGCG combined with ADM arrested cells in S/G2 phase of the cell cycle in a dose-dependent manner.Conclusion EGCG combined with ADM can induce the apoptosis of BEL-7404/ADR cells and block them in S/G2 phase. |
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| Keywords: | (-)-epigallocatechin gallate Hepatocellular carcinoma Apoptosis In vivo |
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