| 大鼠全脑缺血-再灌注损伤后APC-Cdh1蛋白的表达 |
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| 引用本文： | 陈志则,万里,祁月红,姚文龙,邱瑾,张传汉. 大鼠全脑缺血-再灌注损伤后APC-Cdh1蛋白的表达[J]. 中国康复理论与实践, 2010, 16(4): 339-341 |
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| 作者姓名： | 陈志则 万里 祁月红 姚文龙 邱瑾 张传汉 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院麻醉学教研室，湖北武汉市 430030。 |
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| 摘 要： | 目的探讨大鼠全脑缺血-再灌注损伤后APC-Cdh1蛋白的表达变化。方法雄性SD大鼠60只，体重280～350 g，随机分成假手术组和缺血-再灌注组。采用改良Pulsinelli 4-VO法建立SD大鼠全脑缺血模型，在损伤后不同时间点，免疫组化染色检测Cdh1的表达部位并且采用Western blotting检测大鼠海马组织APC-Cdh1蛋白的表达。结果免疫组化检测显示APC-Cdh1在海马区及皮层区中均有大量表达。与假手术组相比，缺血-再灌注组术后第1天APC-Cdh1蛋白表达减少，术后第3天升高(P＜0-05)，术后第7天又降低。结论APC-Cdh1可能与中枢神经系统损伤有着密切的关系。
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| 关 键 词： | 脑缺血 海马 细胞周期后期分裂促进复合物 |
| 收稿时间： | 2010-01-15 |
Expression of APC-Cdh1 Protein after Cerebral Ischemia-reperfusion Damage in Rats |
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| CHEN Zhi-ze,WAN Li,QI Yue-hong,et al.. Expression of APC-Cdh1 Protein after Cerebral Ischemia-reperfusion Damage in Rats[J]. Chinese Journal of Rehabilitation Theory and Practice, 2010, 16(4): 339-341 |
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| Authors: | CHEN Zhi-ze WAN Li QI Yue-hong et al. |
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| Affiliation: | Department of Anesthesiology, Tongji Hospital of Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, Hubei, China |
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| Abstract: | Objective To investigate the expression of APC-Cdh1 protein after cerebral ischemia-reperfusion injury.Methods 60 male Sprague-Dawley rats were randomly divided into Sham-operated group(SH) and ischemia-reperfusion group(IR). The rats of ischemia-reperfusion groups were induced by four-vessel occlusion (4-VO). At different times after injury,the expression of APC-Cdh1 of rat hippocampus was observed by Western blotting and immunohistochemistry.Results Compared with sham-operated group,the expression of Cdh1... |
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| Keywords: | brain ischemia hippocampus anaphase promoting complex |
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