蛋白酪氨酸磷酸酶参与促发的细胞移动过程 |
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引用本文: | 王凤玫,傅志超,徐静.蛋白酪氨酸磷酸酶参与促发的细胞移动过程[J].肿瘤研究与临床,2008,20(12):796. |
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作者姓名: | 王凤玫 傅志超 徐静 |
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作者单位: | 1. 南京军区福州总医院妇产科;2. 南京军区福州总医院放疗科; |
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摘 要: | 目的 探讨蛋白酪氨酸磷酸酶SHP-2在IL-1β所促发的细胞移动中的作用。方法 构建重组质粒SHP-2-GFP以及SHP-2C>S-GFP 并分别转入MCF-7乳腺癌细胞中,建立SHP-2-GFP- MCF-7和SHP-2C>S-MCF-7细胞株。采用荧光显微镜技术观察细胞移动情况;RT-PCR、免疫印迹法检测钙黏蛋白和金属蛋白酶的变化情况,确定蛋白磷酸酶SHP-2在细胞移动中的作用。结果 E-钙黏蛋白在IL-1β刺激的SHP-2转染的乳腺癌细胞株中的表达量是最低的,而金属蛋白酶MMP-9的表达量最高。结论 SHP-2参与IL-1β促发的细胞移动,并且通过减少E-钙黏蛋白的表达以及增强金属蛋白酶MMP-9的分泌来发挥作用的。
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关 键 词: | 蛋白酪氨酸磷酸酶 钙黏着糖蛋白类 基质金属蛋白酶9 白细胞介素 |
收稿时间: | 2008-6-24 |
The tyrosine phosphatase SHP-2 participated in the cell migration induced by IL-1β |
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Abstract: | Objective To investigate whether the tyrosine phosphatase SHP-2 participate in the cell migration induced by IL-1β. Methods Two different reorganized plasmids transfected into the MCF-7, and achieved two different cell lines: SHP-2-GFP-MCF-7 cell lines, SHP-2C>S-GFP-MCF-7 cell lines. The cell migration was detected by a fluorescence microscope and the expression of E-cadherin and MMP-9 were detected by western blotting analysis and RT-PCR. Results The SHP-2-GFP-MCF-7 cell lines showed a higher cell migration ability, and the expression of E-cadherin in the SHP-2-GFP-MCF-7 cell lines was especially lower, nearly undetectable. But in this group the expression of MMP-9 was the highest. Conclusion The tyrosine phophatase SHP-2 participate in the cell migration induced by IL-1β, and maybe produce a marked effect by reinforcing the expression of MMP-9 and reducing the expression of E-cadherin. |
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Keywords: | Cadherin Matrix metalloproteinase 9 Interleukin-1 beta |
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