防风多糖的抗氧化活性研究

目的研究防风多糖的抗氧化活性.方法采用水提醇沉法提取防风多糖,利用CTAB沉淀法对防风多糖进行分离,得到酸性防风多糖(A-SPS)和中性防风多糖(N-SPS).在体外化学模拟条件下,研究不同防风多糖的总还原能力以及对超氧阴离子(O-2 ·)、羟基自由基(·OH)、1,1-二苯基-2苦苯肼自由基(DPPH·)的清除作用和对Fe2-诱导的脂质过氧化反应的抑制作用.结果防风多糖具有一定的清除自由基、抑制脂质过氧化的能力,其中对·OH和DPPH·具有较强的清除作用.在几种防风多糖中,以A-SPS的效果较好,当实验浓度为8 mg/ml时,对·OH和DPPH·的清除率均接近于70%.结论A-SPS具有较强的抗氧化作用,可能是防风多糖的主要活性组分,可作为潜在天然抗氧化剂应用于食品和医药工业中.

Studies on the Antioxidative Activity of Polysaccharides from Radix Saposhnikoviae

OBJECTIVE: To research the antioxidant activity of polysaccharides from Saposhnikovia divaricata (Turez.) Schischk. METHOD: Polysaccharides from Saposhnikovia divaricata (Turez.) Schischk were obtained through hot water extracting and ethanol precipitating method. Using CTAB as precipitant, Saposhnikovia polysaccharide was separated into acid Saposhnikovia polysaccharide (A-SPS) and neutral Saposhnikovia polysaccharide (N-SPS). The total reducing power, scavenging effect to superoixide anion (O2-*), hydroxyl radical (*OH), 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH*) and inhibiting effect to lipid-peroxidation induced by Fe2+ of different Saposhnikovia polysaccharides were measured in vitro chemical simulated systems for evaluating their antioxidant activity. RESULTS: Saposhnikovia polysaccharides had some functions of scavenging free radicals and inhibiting lipid peroxidation, and scavenging effects on DPPH* and OH* were especially significant. A-SPS had better effect among different Saposhnikovia polysaccharides, and the scavenging rate of DPPH* and OH* could approach to 70% when the experimental concentration was 8 mg/ml . CONCLUSION: A-SPS, potential main active ingredient of Saposhnikovia polysaccharide, has strong antioxidant activity and can be applied as a potential natural antioxidant in food and medicine industry.