4种精子计数方法的比较

目的:评价血细胞计数池、Makler计数池、CellVU计数池和计算机辅助精液分析(CASA)系统4种精子计数方法的准确性和精确性。方法:使用两种已知浓度的质控乳胶珠溶液,1份浓度为(35±5)×106/ml,另1份浓度为(18.0±2.5)×106/ml,评价4种计数方法的准确性和精确性,并比较4种方法计数精液的结果。结果:计数乳胶珠时,CellVU计数池的结果最接近已知浓度,分别为(39.70±4.76)、(19.09±2.02)×106/ml,变异系数(CV)值分别为12.80%和10.58%;血细胞计数池和Makler计数池结果均高于已知浓度,前者为(44.84±4.86)×106/ml、(21.04±1.87)×106/ml,CV值分别为10.81%和8.89%,后者为(52.36±7.78)×106/ml、(24.54±3.67)×106/ml,CV值分别为14.86%和14.96%;CASA系统结果低于已知浓度,为(28.53±2.06)、(14.62±0.95)×106/ml,但CV值最低,分别为7.22%和6.50%。计数精液时,CellVU计数池与CASA系统结果差异无显著性(P=0.71),分别为(45.28±34.52)、(41.96±31.93)×106/ml,血细胞计数池和Makler计数池结果差异无显著性(P=0.14),分别为(76.98±59.90)、(63.89±53.84)×106/ml,CellVU计数池、CASA系统与血细胞计数池、Makler计数池结果间差异显著(P<0.05或P<0.01)。结论:计数精液时,CASA系统与CellVU计数结果差异无显著性。各实验室可选择合适的手工或CASA计数方法。

Comparison of Four Methods for Sperm Counting

OBJECTIVE: To evaluate the accuracy and precision of 4 methods including Hemacytometer, Makler chamber, Cell-VU chamber, and computer-aided semen analysis for determining sperm concentration. METHODS: Latex bead solutions with concentrations known as (35 +/- 5) x 10(6)/ml and (18.0 +/- 2.5) x 10(6)/ml and semen samples (n = 54) were counted by the above 4 methods and the results were then compared. RESULTS: Mean bead concentrations for Hemacytometer, Makler, Cell-VU chambers and CASA were (44.84 +/- 4.86), (52.36 +/- 7.78), (39.70 +/- 4.76), (28.53 +/- 2.06) x 10(6)/ml respectively for the standard solution containing (35 +/- 5) x 10(6)/ml, and (21.04 +/- 1.87), (24.54 +/- 3.67), (19.09 +/- 2.02), (14.62 +/- 0.95) x 10(6)/ml respectively for a standard solution containing (18 +/- 2.5) x 10(6)/ml. The results of Cell-VU chamber were consistently similar and close to the standard solutions, while those of Hemacytometer, Makler chambers were overestimated, and those of CASA were underestimated. The coefficients of variation for Hemacytometer, Makler, Cell-VU chambers and CASA were 10.81%, 14.86%, 12.80%, and 7.22% respectively for a higher standard solution, while 8.89%, 14.96%, 10.58%, and 6.50% respectively for a lower standard solution. CASA has the lowest CV%. When semen samples were counted, the results of Hemacytometer, Makler, Cell-VU chambers and CASA were (76.98 +/- 59.90), (63.89 +/- 53.84), (45.28 +/- 34.52), (41.96 +/- 31.93) x 10(6)/ml respectively. There wasn't any significant difference either between Cell-VU chamber and CASA (P = 0.71), or between Hemacytometer and Makler chamber (P = 0.14), while there was significant difference between Cell-VU chamber or CASA and Hemacytometer or Makler chamber (P < 0.05 or P <0.01). CONCLUSION: When counting semen sample, there wasnt any significant difference between Cell-VU chamber and CASA. Each laboratory can select its own proper method for manual or computer-aided analysis.