| 壳聚糖固定化酵母蔗糖酶的研究 |
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| 引用本文： | 马中良,李艳利,焦吉祥,邹媛媛,鲍真真,王旻. 壳聚糖固定化酵母蔗糖酶的研究[J]. 药物生物技术, 2005, 12(6): 379-382 |
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| 作者姓名： | 马中良 李艳利 焦吉祥 邹媛媛 鲍真真 王旻 |
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| 作者单位： | 1. 中国药科大学,生命科学与技术学院,江苏,南京,210009
2. 南京医科大学生化与分子生物学系,江苏,南京,210029 |
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| 摘 要： | 用壳聚糖作吸附剂、戊二醛作交联剂,对酵母蔗糖酶进行固定化研究,同时用琼脂糖固定化的蔗糖酶和游离酶与其进行比较。结果表明,壳聚糖固定化蔗糖酶贮藏稳定性、对变性剂以及对温度的耐受性均明显优越,而Km值与琼脂糖固定化酶相当,但比游离酶明显增高,酶的最适pH三者相近。研究表明,壳聚糖固定化酶所需的戊二醛浓度为0.6%,交联时间不低于6 h。固定化蔗糖酶对变性剂(乙醇、脲)的耐受力明显高于游离酶。
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| 关 键 词： | 酵母蔗糖酶 壳聚糖 固定化 |
| 文章编号： | 1005-8915（2005）06-0379-04 |
| 收稿时间： | 2004-06-29 |
| 修稿时间： | 2004-06-292005-09-23 |
Study on the Properties of Chitsan-immoblized Yeast Sucrase |
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| MA Zhong-liang,LI Yan-li,JIAO Ji-xiang,ZOU Yuan-yuan,BAO Zhen-zhen,WANG Min. Study on the Properties of Chitsan-immoblized Yeast Sucrase[J]. Pharmaceutical Biotechnology, 2005, 12(6): 379-382 |
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| Authors: | MA Zhong-liang LI Yan-li JIAO Ji-xiang ZOU Yuan-yuan BAO Zhen-zhen WANG Min |
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| Affiliation: | 1. The School of Life science and biotechnology, China Pharmaceutical University, Nanjing, 210009, China;2. Department of Biochemisty and Molecular Biology, Nanjing Medical University, Nanjing 210029, China |
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| Abstract: | Yeast sucrase immobilized to chitosan with glutaric dialdehyde as cross-linking agent was studied,while agrose-immobilized and free enzymes were as control.The results showed the stability of chitosan-immobilized enzyme increased much more than that of agarose-immobilized enzyme or free enzyme.The Michaelis-Menten Kinetics of chitosan-immobilized enzyme was equal to that of agarose-immobilized enzyme,but much higher than that of free enzyme.The optimum pH of these enzymes was almost the same.In preparation chitosan-immobilized enzyme,0.6% glutaric dialdehyde and more than 6 hr crosslinking were required.To free enzyme,chitosan-immobilized enzyme was more stable in ancohol or urea. |
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| Keywords: | Yeast sucrase Chitosan Immobilization |
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