七叶皂苷钠预防大鼠术后肠粘连的机制研究

目的探讨七叶皂苷钠预防大鼠术后肠粘连的作用机制。方法建立大鼠肠粘连模型,按随机数字表法将其随机分为模型组、地塞米松组（地塞米松5 mg/kg）和七叶皂苷钠组（七叶皂苷钠2 mg/kg）,每组10只;另随机取10只大鼠开腹后立即缝合作为假手术组。均经尾静脉注射给药,造模前1 d给药1次,术后继续给药3 d,1次/d。术后1周进行粘连程度分级,并取粘连肠段测定羟脯氨酸水平;取血清用ELISA法检测肿瘤坏死因子-α（TNF-α）、白介素（IL）-1β和IL-6的表达。结果与模型组比较,七叶皂苷钠能明显改善肠粘连程度（P〈0.01）,能显著降低粘连肠段羟脯氨酸水平（P〈0.01）,可明显降低血清TNF-α、IL-1β和IL-6水平（P〈0.01）。结论从本研究的初步结果看,七叶皂苷钠通过抑制炎症因子的过度表达,减轻炎症反应,有效预防术后肠粘连的发生。

Mechanism of Sodium Aescinate in Preventing Postoperative Intestinal Adhesion in Rats

Objective To investigate the anti-inflammatory mechanism of sodium aescinate in preventing postoperative intestinal adhesion in rats. Methods The SD rats were subjected to operation for establishing intestinal adhesion models, then randomly divided into model group, dexamethasone group （dexamethasone, i.v. 5 mg/kg）, and sodium aescinate group （sodium aescinate, i.v. 2 mg/kg）, 10 rats in each group. Another ten normal rats were selected as sham operation group. One times administration was administered on day 1 before establishing adhesion model, and administration for 3 d after modeling, once a day. On day 7 after operation, all of the rats were killed. The intestinal adhesion was graded and the adhesive tissues were taken for hydroxyproline determination. The levels of tumor necrosis factor （TNF）-α, interleukin （IL）-19, and IL-6 in the serum were detected by ELISA. Results Compared with the model group, sodium aescinate could obviously improve the severity of postoperative adhesion, markedly decrease hydroxyproline content in the adhesive tissues （P〈 0. 01）, and significantly inhibit the levels of TNF-α, IL-1β, and IL-6 in the serum （P〈0. 01）. Conclusion Sodium aescinate could effectively prevent the formation of postoperative intestinal adhesion by inhibiting the expressions of inflammatory cytokines and decreasing the inflammatory response.