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Identification of Common Edible and Medicinal Mushrooms by DNA Barcoding |
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| Authors: | Yan-Yan Su Huan Tang Xi-Wen Li Wei Sun Zeng-Hua Chen and Li Xiang |
| Institution: | a.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China b.Huaibei Normal University, Huaibei 235000, China,a.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,a.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,a.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,c.Beijing Qianjunfang Academy of Sciences, Beijing 100007, China and a.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
| Abstract: | Objective:To identify common edible and medicinal mushrooms using the internal transcribed spacer(ITS) region.Method:A total of eighty-five samples belonging to forty-three species were used in this study.ITS regions were amplified and sequenced.All ITS regions were analyzed using BLAST and MEGA 5.10 methods.Results:Forty species were identified correctly via BLAST method whereas forty-one species were distinguished via Neighbor joining(NJ)tree.All the species could be distinguished by BLAST combined with NJ tree and ITS sequence characteristics.Conclusion:Forty-three common species of edible and medicinal mushrooms were effectively identified using DNA barcoding technology based on ITS region. |
| Keywords: | Edible mushroom Medicinal mushroom ITS DNA Barcoding |
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