趋化因子受体CXCR4在肝细胞癌表达及肺转移中作用的研究 |
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引用本文: | 薛同春,叶胜龙,陈荣新,韩丹,孙瑞霞,陈洁,汤钊猷.趋化因子受体CXCR4在肝细胞癌表达及肺转移中作用的研究[J].中华肝胆外科杂志,2008,14(5). |
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作者姓名: | 薛同春 叶胜龙 陈荣新 韩丹 孙瑞霞 陈洁 汤钊猷 |
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作者单位: | 复旦大学中山医院,肝癌研究所,上海,200032 |
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基金项目: | 国家重点基础研究发展计划(973计划) |
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摘 要: | 目的 以人肝细胞癌高、低肺转移潜能细胞株MHCC97-H、MHCC97-L为研究对象,研究趋化因子受体CXCR4在肝细胞癌肺转移过程中的作用和意义.方法 RT-PCR和Western blotting比较MHCC97-H、MHCC97-L细胞株CXCR4 mRNA及蛋白质水平的表达.CXCR4配体SDF-1α及肺提取物趋化实验和抗体抑制实验观察SDF-1α和裸鼠肺组织匀浆液对MHCC97-H的趋化迁移作用.结果 趋化因子受体CXCR4在MHCC97-H细胞株表达低于低肺转移潜能细胞株MHCC97-L,蛋白质水平与mRNA水平一致.SDF-1α对MHCC97-H趋化实验表明在1~100 ng/ml浓度范围内均有趋化作用,在浓度为50 ng/L时趋化作用最显著(P<0.05).肺提取物亦发现对MHCC97-H有趋化作用,作用强于MHCC97-L及DMEM组(P<0.05).但加入CXCR4抗体后其趋化作用并未明显下调.结论 CXCR4在肝癌组织及细胞表达并可能参与肝癌肺转移,但并非肝癌细胞趋化转移的主要受体分子.
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关 键 词: | 癌 肝细胞 受体 趋化因子 转移 肿瘤 |
Role of chemokine receptor CXCR4 in lung metastasis of human hepatocellular carcinoma |
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Abstract: | Objective To study the role of chemokine receptor CXCR4 in lung metastasis of hepatocellular carcinoma(HCC)through human HCC cell line MHCC97-H and MHCC97-L with different lung metastasis potentials.Methods The mRNA and protein expression of CXCR4 between MHCC97-H and MHCC97-L by were determined bv RT-PCR and Western blotting respectively.Chemotaxis and antibody inhibition assays were used to observe the chemotaxis effects of SDF-1α,specific ligant of CXCR4, and lung crude extraction from nude mice on MHCC97-H.Results Chemokine receptor CXCR4 in MHCC97-H was expressed lower than that in MHCC97-L,and protein level was in consistent with the mRNA level.MHCC97-H could respond to SDF-1 αbetween 1 ng/ml to100 ng/ml,particularly at 50 ng/L(P<0.05).Lung extraction could have chemotaxis to MHCC97-H,compared with other groups(P<0.05).However,the chemotaxis effect decreased little when being added with CXCR4 antibody.Conclusion Chemokine receptor CXCR4 may participate in the lung metastasis of HCC but is not the major chemokine receptor involved. |
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Keywords: | CXCR4 |
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