婴肝综合征HCMV感染临床与肝功能损害相关性研究

目的:探讨人巨细胞病毒(HCMV)感染与肝功能损害的相关性。方法:采用直接酶标化学发光定量斑点杂交技术测定患儿尿HCMVDNA,并与血清ELISA法检测进行对比研究,用速率法检测肝功能。结果:30例婴肝患 儿血中ELISA法检测HCMV抗体阳性,占16.67％,尿定量斑点杂交检测阳性,占56.67％,HCMV感染和肝功能损害,二者是显著正相关(r=0.9219,P<0.001),尿中阳性率越高,肝脏损害程度越重。结论:随着核酸技术的进展,方法愈先进,检出阳性率愈高,证实HCMV是婴肝综合征的重要病原,对有肝功能损害者,要想到可能存在HCMV的感染。

Correlation between HCMV Infection and Hepatic Damage in Infants with Infant Hepatitis Syndrome

Objective: To investigate the correlation between human cytomegalovirus infection and hepatic damage. Methods: The urine specimen of infants with infant hepatitis syndrome was detected for HCMVDNA by Enzyme Labeled Chemiluminescence Dot Hybridization and the results were contrasted with that by ELISA method. Liver function was detected by speed method. Results: The positive rate of HCMV-antibody in serum of thirty infants with infant hepatitis syndrome by ELISA was 16.67% and that in urine specimen by dot hybridization was 56.67% . There is markedly correlation between HCMV infection and hepatic damage (r = 0.9219,P<0.001). The higher positive rate of HCMV DNA in urine, the more severe hepatic damage. Conclusion: HCMV infection is the leading factor in etiology of infant hepatitis syndrome. For those infants with hepatitis syndrome accompanied by hepatic damage, HCMV infection should be considered.