基质金属蛋白酶及其抑制剂在边缘系统胶质瘤侵袭中的作用

 目的 探讨基质金属蛋白酶(MMP-2、MMP-9)及其组织抑制因子(TIMP-2)在边缘系统胶质瘤侵袭中的作用。方法 利用免疫组织化学技术SP法检测MMP-2、MMP-9及TIMP-2在35例高、低级别胶质瘤和20例脑良性肿瘤(脑膜瘤)中的表达。结果 ①Ⅲ～Ⅳ级胶质瘤MMP-2、MMP-9蛋白的表达明显高于Ⅰ～Ⅱ级，Ⅰ～Ⅱ级胶质瘤MMP-2、MMP-9蛋白的表达明显高于脑膜瘤，其两两之间比较具有显著性差异。②高级别胶质瘤组TIMP-2蛋白表达明显低于低级别组，低级别组TIMP-2蛋白表达明显低于脑膜瘤组，其两两之间比较具有显著性差异。③35例胶质瘤和20例脑膜瘤中MMP-2与MMP-9呈正相关(y=0．86，P<0．01)，MMP-2与TIMP-2呈负相关(γ=-0．65，P<0．01)，MMP-9与TIMP-2呈负相关(γ=-0．58，P<0．01)。结论 ①MMP-2、MMP-9蛋白的表达与胶质瘤的恶性程度有关，可能成为胶质瘤恶性程度、侵袭能力和预后的判断指标。②TIMP-2是MMP-2的抑制因子，两者之间失衡是促进胶质瘤侵袭的重要因素之一。

The Function of MMP-2﹑MMP-9 and TIMP-2 in Human Brain Glioma Invasion

Objective 　To investigate the function of matrix metalloproteinase-2 (MMP-2) , matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of metalloproteinase-2 ( TIMP-2) in human brain limbic system glioma invasion. Methods 　The expression of MMP-2 ,MMP-9 and TIMP-2 in low-grade and high-grade glioma and meningioma was studied via the immunohistochemical stain. Results 　① In high malignant glioma , the expressions of MMP-2 and MMP-9 were significantly higher than those in low malignant glioma ( P < 0. 05 and P < 0. 01 respectively) . In low malignant glioma , the levels of MMP-2 and MMP-9 were significantly higher than those in meningioma ( P < 0. 05 and P < 0. 01 respectively) . There were significant differences of MMP-2 and MMP-9 levels among in high-grade ,low-grade glioma and meningioma. ② TIMP-2 expression level was especially low in gliomas of grade Ⅲand grade Ⅳ,but significantly higher in gliomas of grade Ⅰand grade Ⅱ,particularly in meningiomas. ③ There was a positive correlation between MMP-2 and MMP-9 levels ( r = 0. 86 , P < 0. 01) ; negative correlation between MMP-2 and TIMP-2 levels , negative correlation between MMP-9 and TIMP-2 levels ( r = 0. 58 , P < 0. 01) in 35 gliomas. Conclusion 　These result s suggest that : ① the expressions of MMP-2 and MMP-9 in human brain glioma are related to malignant progression of gliomas ; their expression levels may become an assessable factor for malignancy and invasion of glioma and prognosis of patient ; ②a balance between MMP-s and TIMP-s has an important role to play in human brain gliomas , their unbalance may be a main factor in enhancing glioma invasion. TIMP expression may be valuable markers for tumor malignancy.