银胶菊内酯对PGCL3细胞uPA活性和表达的影响

目的:研究银胶菊内酯对人肺癌细胞PGCL3尿激酶型纤溶酶原激活物(uPA)蛋白表达及活性的影响,探讨其抗肿瘤的作用机制.方法:MTT法检测银胶菊内酯对PGCL3细胞的增殖抑制作用;发色底物法检测银胶菊内酯对PGCL3细胞分泌的uPA活性的影响;Western blot法检测银胶菊内酯对uPA表达的影响.结果:5～160μmol/L的银胶菊内酯作用PGCL3细胞24 h后,明显抑制PGCL3细胞的增殖,IC50值为17.6 μmol/L;5、10、20 μmol/L银胶菊内酯明显降低PGCL3细胞分泌的uPA活性;银胶菊内酯还能降低PGCL3细胞uPA蛋白表达.结论:银胶菊内酯抗肿瘤活性与uPA蛋白表达及活性有关.

Effects of Parthenolide on the Activity and Expression of Urokinase Type Plasminogen Activator in PGCL3 Cells

OBJECTIVE: To study the effect of Parthenolide on the activity and expression of urokinase type plasminogen activator (uPA) on PGCL3 cells and to investigate the antitumor mechanisms of Parthenolide. METHODS: MTT assay was used to determine the growth inhibition by Parthenolide in PGCL3 cells; Effect of Parthenolide on the activity of uPA secreted by PGCL3 cells was measured by chromogenic substrate assay;The expression level of uPA protein was assessed by Western blot analysis. RESULTS: Parthenolide could inhibit proliferation of PGCL3 cells after 24h treatment, the IC50 value was 17.60 micromol/L; Parthenolide reduced significantly the activity of uPA secreted by PGCL3 cells and down-regulated the expression level of uPA protein. CONCLUSION: The antitumor activity of Parthenolide is involved in the activity and expression of urokinase type plasminogen activator.