| 两个不同厂家试剂盒检测EB病毒EA-IgA、VCA-IgA的比较 |
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| 引用本文： | 罗玲,曾桦,罗晓红.两个不同厂家试剂盒检测EB病毒EA-IgA、VCA-IgA的比较[J].中国实用医药,2011,6(15):7-8. |
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| 作者姓名： | 罗玲 曾桦 罗晓红 |
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| 作者单位： | 中山大学孙逸仙纪念医院检验科,广州,510120 |
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| 摘 要： | 目的 比较分析两个不同厂家试剂检测EB病毒早期抗原IgA抗体(EA-IgA)、衣壳抗原IgA抗体(VCA-IgA),并做方法学评价.方法 分别用两个不同厂家的ELISA试剂盒检测184例鼻咽癌(NPC)患者及184例正常人的血清EA-IgA和VCA-IgA.结果 与欧蒙公司的试剂盒相比,IBL公司的试剂EA-IgA的阳性符合率为66.07%,阴性符合率为89.45%,总符合率为82.34%,Kappa值为0.57;VCA-IgA的阳性符合率为67.57%,阴性符合率为99.32%,总符合率为80.16%,Kappa值为0.62.结论 两个厂家的试剂盒在分别验证EA-IgA,VCA-IgA时,具有高度一致性.国内IBL公司生产的试剂可替代进口试剂.
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| 关 键 词： | EB病毒 鼻咽癌 |
Comparison of the two kits from two different manufacturers in detecting EA-IgA and VCA-IgA of Epstein-Barr virus |
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| LUO Ling,ZENG Hua,LUO Xiao-hong.Comparison of the two kits from two different manufacturers in detecting EA-IgA and VCA-IgA of Epstein-Barr virus[J].China Practical Medical,2011,6(15):7-8. |
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| Authors: | LUO Ling ZENG Hua LUO Xiao-hong |
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| Institution: | LUO Ling,ZENG Hua,LUO Xiao-hong.The Sun Yat-sen Memorial Hospital of Sun Yat-sen University,GuangZhou 510120,China |
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| Abstract: | Objective To evaluate the two kits from two different manufacturers in detecting EA-IgA and VCA-IgA of Epstein-Barr virus,then provide the methodology evaluation.Methods The serum of EBVEA-IgA and VCA-IgA in 184 Nasopharyngeal patients and 184 healthy adults were detected use two kits.Results Compared with the kit of EUROIMMUN,for the kits of IBL to detect EA-IgA,the positive coincidence rate was 66.07%,negative coincidence rate was 89.45% and the total coincidence rate was 82.34%,with Kappa value of 0.57;f... |
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| Keywords: | EA-IgA VCA-IgA |
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