循环内皮干细胞与症状性颅内动脉狭窄关系研究

目的研究外周血内皮干细胞（cEPC）数量与颅内动脉狭窄出现及严重度的关系。方法本研究包括了36名颅内动脉狭窄患者（病例组）,40名无脑动脉狭窄卒中患者（病例对照组）和36名健康志愿者（健康对照组）。外周血内皮干细胞基于CD34、CD133和KDR抗原的表达,应用流式细胞仪确定为CD34（＋）CD133（＋）KDR（＋）细胞。结果颅内大动脉狭窄患者外周血内皮干细胞数量显著高于卒中无狭窄患者（P〈0.01）和健康志愿者（P〈0.01）,而卒中无狭窄患者和健康志愿者间内皮干细胞数量差异无统计学差异（P〉0.05）。狭窄率≥70%患者cEPC数量显著高于〈70%患者（0.091±0.035%vs 0.052±0.012%,P〈0.01）。单支狭窄、多支狭窄两组间比较,cEPC数量无显著差异（P〉0.05）。三组中,内皮干细胞数量与纤维蛋白原浓度均无相关性。结论内皮干细胞数量异常增加可能在颅内动脉狭窄发生发展中发挥重要作用。

Circulating endothelial progenitor cells in patients with sympotic intracranial artery stenosis

Objective To determine whether the number of EPC in peripheral blood was associated with the presence and severity of intracranial large-artery stenosis.Methods A total of 112 individuals were examined,including 36 patients with intracranial artery senosis（IAS）,40 patient controls with no stenotic cerebral artery and 36 healthy controls.Circulating EPC（cEPC） were defined by the surface markes CD34/CD133/KDR and analyzed by flow cytometry.Results The number of cEPC was significantly higher in patients with IAS than that in patient controls（P0.01） and healthy controls（P0.01）.However,no significant difference was found in cEPC between the two controls.The cEPC was significantly higher in patients with ≥70% than with 70% stenosis（0.091±0.035% vs 0.052±0.012%,P0.01）.There was no significant difference in the cEPC between single and multiple stenosis patients（P0.05）.No significant correlation between cEPC numbers and plasm levels of fibrinogen was found in the three groups（all P0.05）.Conclusion Our results show increased EPC numbers may play a role in the presence and development of intracranial artery stenosis.