Anti-Oxidant Effects of Pomegranate Juice on Saccharomyces Cerevisiae Cell Growth |
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Authors: | Abdullah Aslan Muhammed ?smail Can Didem Boydak |
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Institution: | Firat University, Faculty of Science, Department of Biology, Elazığ-TURKEY |
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Abstract: | BackgroundPomegranate juice has a number of positive effects on both human and animal subjects.Material and methodsFour groups were used in this study. i: Control group, ii: H2O2 group, iii: Pomegranate juice (PJ) group and iv: PJ + H2O2 group. Following the sterilization method for pomegranate juice (10%) and H2O2 (6% v/v), Saccharomyces cerevisiae cultures were added and the cultivation incubated at 35°C for 72 hours. Fatty acids and vitamin concentrations were measured using HPLC and GC and the total protein bands profile were determined by SDS-PAGE.ResultsAccording to our results statistically significant differences have been determined among the study groups in terms of fatty acids and vitamin (p<0,05). Fatty acid synthesis, vitamin control and cell density increased in groups to which PJ was given in comparison with the control group (p<0,05). Pomegranate juice increased vitamins, fatty acids and total protein expression in Saccharomyces cerevisiae in comparison with the control.ConclusionPomegranate juice has a positive effect on fatty acid, vitamin and protein synthesis by Saccharomyces cerevisiae. Accordingly, we believe that it has significantly decreased oxidative damage thereby making a positive impact on yeast development. |
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Keywords: | Pomegranate juice SDS-PAGE fatty acid vitamin |
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