Extraction and Isolation of Alkaloids of Sophora Alopecuroides and Their Anti-Tumor Effects in H22 Tumor-Bearing Mice |
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Authors: | AiHua Chang Zhen Cai ZhiHong Wang ShengKai Sun |
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Affiliation: | 1.Affiliated Hospital of Logistics College of the CAPF, Tianjin 300162, China;2.Tianjin First Centre Hospital,Tianjin,300192,China |
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Abstract: | BackgroundAlkaloids of Sophora alopecuroides have good biological activity, and are widely used in clinical settings, which not only have pharmacological activities of anti-cancer, cancer suppression, as well as the inhibition, and killing of various microorganisms; but also possess extensive pharmacological effects on immune system, nervous system and cardiovascular system. The objective of this paper was to extract and isolate total alkaloids of Sophora alopecuroides (TASA), and to study their anti-tumor effects in H22 tumor-bearing mice.Materials and MethodsTASA were extracted and isolated using thin-layer chromatography, and column chromatography; and the isolated compounds were analyzed using nuclear magnetic resonance. The inhibitory effects of TASA on tumor in H22-bearing mice were determined by MTT assay.ResultsThree compounds were isolated from Sophora alopecuroides L., which were matrine, oxymatrine and sophoridine, respectively. Meanwhile, mouse H22 sarcoma model was established and different doses of TASA apparently inhibited solid H22-tumor in mice; it inhibited the thymus, and spleen to some extent; the degree of inhibition was more obvious for the spleen.ConclusionTASA has an anti-tumor effect in H22 tumor-bearing mice. |
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Keywords: | Sophora alopecuroides L. H22 tumor-bearing mice matrine oxymatrine and sophoridine |
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