反胶束液—液萃取牛血清白蛋白的动力学研究：I.萃取过程

以十六烷基三甲基溴化铵－正己醇－正辛烷反胶束溶液为萃取剂，研究了从水溶液中萃取牛血清白蛋白的动力学。在恒界面池中，通过改变搅拌转速，水相ｐＨ值和离子强度，有机相表面活性剂和助溶剂浓度，测定了在多种条件下的表观传质系数，从而判定萃取过程是由水膜扩散和界面过程黄同控制。计算了扩散的传质分系数，进而获取了界面传质分系数随水相条件的变化情况，表明萃取的界面质传为一协同过程，静电作用影响到界面变形的程度，使

Kinetics of the Solubilization Using Reversed Micelles I.Extraction of Protein

The kinetics of BSA extraction has been studied with CTAE-hexanol-octanereversed mice11ar solution.In order to determine the overall mass-transfer coefficients,astirred transfer cell with constant interface was operated under varied conditions,such as thestirring speed,the pH value and ionic strength in aqueous phase,and the concentration ofsurfactant and cosolvent.The results showed that the extraction is controlled together byinterfacial process and diffusion through the aqueous film.The individual mass transfer coefficients of diffusion in aqueous phase were calculated,so that a series of individual interfacialcoefficient at various aqueous phase conditions could be obtained.It shows that the interfacial transport of BSA occurs via a cooperative mechanism.The interfacial transfer rate isstrongly affected by the electrostatics in protein/interfacial interaction,which determines theextent of deformation at the interface.