Computerized three-dimensional study of a rotavirus model of biliary atresia: comparison with human biliary atresia |
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Authors: | Regina Y Y Chan Carolyn E L Tan Gerard Czech-Schmidt Claus Petersen |
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Institution: | (1) Department of Pediatric Surgery, KK Women’s and Children’s Hospital, 100 Bukit Timah Road, , Singapore, 229899;(2) Department of Pediatric Surgery, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany;(3) Department of Medicine, National University of Singapore, NUH Main Building # 03-31, 5 Lower Kent Ridge Road, , Singapore, 119074 |
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Abstract: | Biliary atresia is a panbiliary disease causing obstructive jaundice in neonates and infants. The clinical spectrum can be
broadly categorized into the fetal and perinatal types. A consistent animal model that accurately mimics the whole clinical
spectrum of biliary atresia is not yet available. However, rotavirus infection of neonatal mice has been shown to produce
atresia in the biliary system. This study investigates the three-dimensional computerized morphology of the murine neonatal
model comparing with age-matched control mice. Newborn Balb/c mice were injected intraperitoneally with rhesus rotavirus within
24–48 h after birth. Control mice received 0.9% NaCl. Pups with symptoms of cholestasis were sacrificed from the 5th to the
15th postinjection day, as were age-matched controls. Their hepatobiliary tissues were prepared for three-dimensional computerized
image reconstruction. Rotavirus infection caused obliteration of the intrahepatic bile ducts and single to multiple atresias
in the extrahepatic bile duct. At 15 days postinjection, intrahepatic ductal proliferation appeared, and the three-dimensional
appearances of the intrahepatic biliary structures were similar to the human disease. Cystic duct and gallbladder dilatation
was frequently seen in this model, and this feature distinguishes it from the human disease in which the gallbladder is almost
always atretic. This rotavirus murine model demonstrates many of the features of human perinatal biliary atresia, and can
be used as an investigative tool to further study the pathogenesis of biliary atresia. |
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Keywords: | Biliary atresia Animal model Rotavirus |
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