复方安替威胶囊血中移行成分的富集工艺研究

目的:研究复方安替威血中移行成分的富集工艺条件及参数。方法:以复方安替威的主要入血成分绿原酸和黄芩苷为考察指标,用HPLC测定含量,确定大孔树脂法富集复方安替威血中移行成分的最佳工艺条件。结果:复方安替威胶囊内容物经水提醇沉后,以NKA-2型大孔树脂为吸附剂,3 BV的水洗脱除去杂质后,收集5 BV的50%乙醇洗脱液为最佳富集工艺;终产物中绿原酸和黄苷芩的含量均较原复方安替威胶囊提高了近5倍。结论:以中药血清药物化学为指导的大孔树脂法,是富集复方安替威血中移行成分的有效方法,也为其他中药复方的精制提供借鉴。

Study on the collecting method of constituents Absorbed into the blood from Anti-V capsule

Objective: Aim To establish the condition and parameters for collecting the effective constituents of Anti-V capsule.Method: Based on HPLC,using chlorogenic acid and baicalin which are the main constituents absorbed in the blood as the marker substance,the best technical process of macropore absorbed resin was evaluated by the quantity of two compounds in the collected part.Result: After the abstraction of the capsule,the collection method of NKA-2 macropore absorbed resin as sorbent and 5 bed volume 50% ethanol as mobile phase was definited as the best craftwork.In the collected part,the content of chlorogenic acid and baicalin was five times than in the capsule.Conclusion:In guide of serum pharmacochemistry,macropore absorbed resin is the effective method to collect the constituents absorbed in blood of Anti-V capsule.At the same time,this method provides a way to research other Compound medicine.