活性氧介导5, 7- 二甲氧基黄酮诱导结肠癌HT- 29 细胞凋亡

目的研究5，7-二甲氧基黄酮（5，7-dimethoxyflavone，DMF）诱导体外培养人结肠癌HT-29细胞凋亡作用及其机制。方法以HT-29细胞为研究对象，利用ELISA法测定细胞DNA断裂，流式细胞术检测细胞凋亡率和活性氧（ROS）水平。结果DMF激活HT-29细胞ROS生成（P〈0．05），呈浓度依赖性。DMF以浓度依赖方式促进HT-29细胞DNA断裂（P〈0．05），同时，诱导细胞凋亡率增高（P〈0．05）；10μmol．L^-1N-乙酰半胱氨酸预孵育30min，能有效阻断DMF诱导ROS生成和细胞凋亡作用（P〈0．05）。结论DFM通过促进结肠癌HT-29细胞ROS生成诱导细胞凋亡。

5, 7-dimethoxyflavone-induced apoptosis of colon cancer HT- 29 cell line mediated by Reactive oxygen species

Objective To investigate the effect of 5, 7-dimethoxyflavone（DMF） on induction of apop- tosis of human colon cancer HT-29 cell line cultured in vitro and its mechanisms. Method HT-29 cells were cultured in vitro.The cell DNA fragmention was determined using ELISA assay. Reactive oxygen spe- cies （ROS） level and apoptotic rate were examined by flow cytomety （FCM）. Results DMF significantly promoted the intracellular ROS generation（P〈0.05） in HT-29 cells, in a concentration-dependent manner. DMF significantly increased the DNA fragmention of HT-29 cells （P〈0.05）, in a concentration-dependent manner. Pretreated with 10 μmol.L^-l N-L-acetylcysteine for 30 rain can effectively blocked the effects of DMF on induction of apoptosis and ROS generation in HT-29 cells. Conclusion DMF induces apopto- sis of human colon cancer HT-29 cell line through generation of ROS.