黄芪多糖对人肺癌A549 细胞增殖的作用及其机制的实验研究

目的探讨黄芪多糖（astragalus polysaccharide,APS）对人肺癌A549细胞增殖的作用及其机制。方法用不同浓度的APS（25μg.mL-1、50μg.mL-1、10μg.mL-1、200μg.mL-1、400μg.mL-1）作用于人肺癌A549细胞,用MTT法检测细胞增殖的抑制率,免疫细胞化学法检测bcl-2和bax的表达。结果 APS的浓度在25μg.mL-1～400μg.mL-1范围内均可抑制人肺癌A549细胞的增殖,100μg.mL-1的APS对A549细胞增殖的抑制率达最高,100μg.mL-1的APS作用于人肺癌A549细胞48和72小时后,均可下调bcl-2和上调bax的表达。结论 APS能够可抑制人肺癌A549细胞的增殖,可能与其下调bcl-2和上调bax的表达有关。

Experimental study on the effect and mechanisms of astragalus polysaccharide on the proliferation of human lung cancer cell line A549

Objective To investigate the effect and mechanisms of APS on proliferation of human lung cancer cell line A549.Method Cells were treated with different concentrations of APS（25μg.mL-1,50 μg.mL-1,100 μg.mL-1,200μg.mL-1,400μg.mL-1）.The inhibitory rate of A549 cell proliferation was detected by MTT and the expression of Bcl-2 and bax were tested by immunocytochemistry.Results APS inhibited the proliferation of A549 cells within 25μg.mL-1～400μg.mL-1.APS at a concentration of 100 μg.mL-1 significantly inhibited the proliferation of A549 cells.The expression of bcl-2 was down-regulated and the expression of bax was up-regulated in A549 cells after treated by the APS at a concentration of 100μg.mL-1for 48 and 72 hours.Conclusion APS was able to inhibit the proliferation of A549 cells,the downregulation of bcl-2 and upregulation of bax may be involved in this effect.