The rapid onset of tolerance to ataxic effects of ethanol in mice |
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Authors: | Edward J. Gallaher Linda M. Parsons Dora B. Goldstein |
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Affiliation: | (1) Department of Pharmacology, Stanford Medical Center, 94305 Palo Alto, CA, USA;(2) VA Medical Center (151 H), 3801 Miranda Avenue, 94304 Palo Alto, CA, USA |
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Abstract: | We have developed a precise quantal method for assessing the sensitivity to ethanol in the mouse. Mice placed on a clamped stationary horizontal dowel are scored ataxi or not ataxic depending on whether they are able to remian on the dowel during a 30-s observation period. A threshold blood ethanol concentration is determined by assaying tail blood drawn immediately upon recovery from ethanol-induced ataxia. This threshold is quite reproducible within a population of Swiss-Webster mice (coefficient of variation 9%). The precision of this method allowed us to follow the onset of rapid tolerance during a series of sequential IP ethanol doses. Tolerance persisted overnight in the absence of ethanol, and was found not to increase further with additional ethanol exposure on 2 subsequent days. The observed tolerance was shown not to be due to circadian changes in ethanol sensitivity or repeated practice on the task, indicating a true tissue tolerance. |
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Keywords: | Alcohol Ethanol Rapid tolerance Ataxia |
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