葎草水提物止痒作用的实验研究

目的：研究葎草水提物的止痒作用。方法：通过采用4-氨基吡啶（4-AP）诱发小鼠皮肤瘙痒反应、氯喹诱发小鼠全身瘙痒反应、组胺致豚鼠局部瘙痒反应及2,4-二硝基氟苯（DNFB）致小鼠右耳廓迟发型变态反应的瘙痒动物模型,并用放射免疫分析方法（RIA）测定氯喹致小鼠瘙痒后血清中IL-4的含量,观察葎草水提物的止痒作用。结果：葎草水提物100、30mg/kg显著抑制4-AP和氯喹诱发小鼠瘙痒反应的持续时间,其中100mg/kg可显著减少搔痒次数,并显著降低氯喹诱发小鼠瘙痒反应后血清中IL-4的含量;葎草水提物25、7.5mg/kg显著提高豚鼠致痒阈;100、30mg/kg显著抑制DNFB致小鼠右耳廓肿胀。结论：葎草水提物具有止痒作用,可能与抑制组胺和IL-4的释放有关。

Experimental study on antipruritic effect of Humulus scandens extract from water

AIM： To study the antipruritic effect of Humulus scandens extract from water. METHODS： The animal models of mice skin-scratching response induced by 4-Animopridine（4-AP）, mice body-scratching behavior induced by chloroquine, guinea pig partial itching reaction induced by histamine, and the right ear auricle delayed type hypersensitivity of mice induced by 2,4-dinitrochlorobenzol（DNFB） were utilized , and the content of IL-4 in mice serum after the body-scratching behavior induced by chloroquine was determined by radio immunoassay（RIA） to observe the antipruritus effect of Humulus scandens extract from water. RESULTS： 100, 30 mg/kg of Humulus scandens extract from water inhibited mice scratching duration induced by 4-AP and chloroquine remarkably; 100 mg/kg of Humulus scandens extract from water decreased scratching frequency induced by 4-AP and chloroquine, and reduced the content of IL-4 in mice serum after the body-scratching behavior induced by chloroquine significantly ; 25,7.5 mg/kg of Humulus scandens extract from water improved itch threshold of guinea pig obviously; 100, 30 mg/kg of Humulus scandens extract from water depressed the right ear auricle of mice swelling induced by DNFB markably. CONCLUSION： Humulus seandens extract from water possesses antipruritic effect, which may be attributed to the action of suppressing release of histamine and IL-4.