苯对骨髓CD34+细胞及单个核细胞凋亡的影响

目的　探讨不同苯浓度作业场所对淋巴细胞微核和 /淋巴细胞百分比高的工人的骨髓CD34 细胞数量及单个核细胞AnnexinV ,Fas阳性表达率的影响。方法　对 2 0例淋巴细胞微核和 /淋巴细胞百分比偏高的苯作业工人和 10例正常人 ,采用流式细胞术检测CD34 细胞数量 ,单个核细胞AnnexinV和Fas的阳性表达率 ;气相色谱法测定作业场所苯浓度。结果　苯浓度 34～ 5 82mg/m3 作业工人骨髓CD34 细胞数量明显低于正常对照组 ,有显著性差异 (P <0 .0 5 ) ,相关分析呈负相关 (P <0 .0 5 ) ,r =- 0 .95。骨髓单个核细胞AnnexinV阳性表达率与Fas阳性表达率明显高于正常对照组 (P <0 .0 5 ) ,相关分析呈正相关 (P <0 .0 5 ) ,r =0 .96 ;苯浓度 8～ 32mg/m3 作业工人骨髓仅CD34 细胞数量高于正常对照组。结论　动态测定苯作业工人骨髓CD34 细胞数量 ,AnnexinV和Fas阳性表达率对慢性职业性苯中毒有早期诊断和预防意义

Effect of benzene on CD34 + cell and apoptosis of mononuclear cell in bone marrow

Objective To study the effect of benzene on the bone marrow CD34 cells number,mononuclear cell Annexin V and Fas expression of positive percentage in the workers whose lymphocyte mirokernel or percentage were higher than normal one.Methods For the 20 workers and 10 normal ones,CD34 cells number,mononuclear cells Annexin V and Fas was detected by flow cytometric analysis,benzene concentration was determined by gas chromatography.Results CD34 cells number in group benzene concentration of 34-582?mg/m 3 was significant lower than that in control group(P<0.05),.CD34 cells number was negative correlated with benzene concentration (P<0.05),r=-0.95.Annexin V positive percentage,expression and Fas were significant higher than that in control group(P<0.05).They were correlated with benzene concentration (P<0.05),r=0.96.CD34 cells number in group benzene concentration of 8-32?mg/m 3 was significant higher than that in control group.Conclusion Dynamic detection of CD34 cells number,Annexin V and Fas expression of positive percentage should be useful for early diagnosis and prevention for workers with professional benzene poison.