葛根素固体脂质纳米粒抗肝损伤作用研究

目的 制备葛根素固体脂质纳米粒(puerarin solid lipid nanoparticles，Pue-SLN)，对Pue-SLN进行体外释药考察，并探讨Pue-SLN对CCl4诱导的急性肝损伤大鼠的治疗作用。方法 采用乳化-超声分散法制备Pue-SLN。大鼠腹腔注射CCl4造成急性肝损伤模型。48只大鼠随机分成6组：正常组、模型组、甘草酸二胺阳性对照组(13.5 mg·kg^-1)、Pue-SLN高浓度组(27 mg·kg^-1)、中浓度组(13.5 mg·kg^-1)、低浓度组(6.75 mg·kg^-1)。分别测定大鼠血清中丙氨酸转移酶(ALT)、碱性磷酸酶(ALP)、天门冬氨酸转移酶(AST)的活力，肝组织匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)含量，计算肝脏指数，并对肝组织进行组织形态学检查。结果 Pue-SLN各剂量组均能抑制肝损伤大鼠血清中ALT、AST、ALP活性，降低肝匀浆中MDA含量，增强SOD、GSH-PX活性，改善肝组织的病理形态。结论 Pue-SLN对CCl4诱导的大鼠急性肝损伤具有治疗作用。

Effects of Puerarin Solid Lipid Nanoparticles on Liver Injury

OBJECTIVE To research the therapeutic effect of puerarin solid lipid nanoparticles(Pue-SLN) on liver injury in rats, prepare Pue-SLN and investigate drug release of Pue-SLN. METHODS Pue-SLN was prepared by emulsification ultrasonic dispersion method. Acute liver injury was induced by intraperitoneal injection of carbon tetrachloride in rats. Rats were randomly divided into 6 groups: normal control group, model group, glycyrrhizinate acid diamine positive control group(13.5 mg·kg^-1), Pue-SLN groups with high concentration(27 mg·kg^-1), middle concentration(13.5 mg·kg^-1) and low concentration (6.75 mg·kg^-1). ALT, ALP, AST in serum and MDA, SOD, GSH-PX in liver tissue homogenate were detected. And then, liver index and obtained histomorphology examination of liver tissue were calculated. RESULTS Pue-SLN groups could significantly inhibit the activities of ALT, AST, ALP in serum, decrease the content of MDA in liver homogenate and increase the activities of SOD, GSH-PX in liver homogenate. Pue-SLN groups could improve the pathological of liver tissue via observing confirm the pathological section histomorphology of liver tissue. CONCLUSION Pue-SLN have therapeutic effects on acute liver injury induced by carbon tetrachloride in rats.