Methodological simplifications in radioimmunoassay of urinary aldosterone. |
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Authors: | R Malvano S Orlandini P Cozzani P Duranti N Simonini A Salvetti |
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Affiliation: | Laboratory of Clinical Physiology, C.N.R., Pisa, Faculty of Medicine, Institute of Chemistry, E.U.L.O., Brescia, S.O.R.I.N. Biomedical Research, Saluggia, and Clinical Medicine, University of Pisa, PisaItaly |
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Abstract: | Simplification of radioimmunoassay procedures of urinary aldosterone-18-glucuronide was attempted, taking into consideration the aspects implied by the hydrolysis of urine and the assay itself. The procedure standardized for the hydrolysis step (samples diluted with a two-fold volume of 0.2 N HCl and incubated at 30 degrees C for 16-24 h) proved suitable in terms of practicability and accuracy. Aldosterone antisera, raised in the rabbit against an aldosterone-3-bovine albumin conjugate, were selected according to their specificity towards competing steroids. Depending on the characteristics of the antisera used, an assay of extracts, or even direct measurements of hydrolyzed urines excluding any extraction, were found to yield reliable results. In the case of a high-quality antiserum, evidence for the adequacy of assay on non-hydrolyzed urine extracts for the measurement of the excretion of unconjugated aldosterone was provided by some preliminary data. The results of the experiments, directed at the methodological and clinical validation of the simplified procedures, are reported and discussed in this paper. |
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Keywords: | Correspondence to: Dr. R. Malvano Laboratory of Clinical Physiology C.N.R. Via Savi 8 56100 Pisa Italy. |
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