| 天麻总DNA提取的研究 |
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| 引用本文： | 李相陵,王晓玲,刘安发.天麻总DNA提取的研究[J].贵阳医学院学报,2005,30(5):399-400. |
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| 作者姓名： | 李相陵 王晓玲 刘安发 |
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| 作者单位： | [1]贵阳医学院药用植物与生药学教研室,贵州贵阳550004 [2]贵州大学植保系,贵州贵阳550025 |
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| 基金项目： | 基金项目国家科技部2004BA721A33、贵州省科技黔科合农社字（2001）1125号、黔科合中药专字[2003]51号资助项目 |
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| 摘 要： | 目的：寻找快速便捷的天麻总DNA的提取方法.方法：采用天麻干品为材料,用CTAB,SDS 2种提取法及液氮、玻璃砂2种研磨方法提取干天麻总DNA,并进行了比较.结果：2种不同的研磨法和2种不同的提取方法,均可从天麻中提取到一定纯度的DNA,其中CTAB法提取的DNA纯度较好,产率较高;SDS法提取的DNA纯度较低且不稳定,产率较低.结论：从天麻干品中采用CTAB法提取DNA用于分子生物学研究是可行的,用玻璃砂研磨能取得与液氮相近的结果.
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| 关 键 词： | 植物 药用 DNA 氮 天麻 玻璃砂 |
| 文章编号： | 1000-2707（2005）05-0399-02 |
| 收稿时间： | 2005-03-08 |
| 修稿时间： | 2005-05-30 |
The Isolation of Total DNA from Rhizoma Gastrodiae |
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| LI Xiang-li,WANG Xiao-li,LIU An-fa.The Isolation of Total DNA from Rhizoma Gastrodiae[J].Journal of Guiyang Medical College,2005,30(5):399-400. |
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| Authors: | LI Xiang-li WANG Xiao-li LIU An-fa |
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| Institution: | 1. Department of Plant Protection, Guizhou University, Guiyang 550025, China; 2. Department of Medical Plantl and Pharmacognosy, School of Pharmacy, Guiyang Medical College, Guiyang 550004, China |
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| Abstract: | Objective: To develop a quick and convenient method for the extraction of total DNA of Rhizoma Gastrodiae.Methods: The isolation methods of CTAB,SDS were used and the results were compared,and the effects of 2 grinding methods were also compared.Results: The DNA in Rhizoma Gastrodiae was extracted successfully with both the two methods.The results of CTAB were better than the that of SDS method.Conclusion: The CTAB method is practicable in the isolation of DNA from dry Rhizoma Gastrodiae.The gringding result of glass-sand is similar with that of nitrogen. |
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| Keywords: | plant medical DNA nitrogen Rhizoma Gastrodiae glass-sand |
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