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| 血管脱细胞细胞外基质制备的实验研究 | |
| 引用本文： | 刘咸罗,孙大宽,钱小星,柳小刚,陈克辉,胡开兵,张培华.血管脱细胞细胞外基质制备的实验研究[J].中国现代普通外科进展,2002,5(1):14-17. |
| 作者姓名： | 刘咸罗 孙大宽 钱小星 柳小刚 陈克辉 胡开兵 张培华 |
| 作者单位： | 1. 安徽省合肥市第二人民医院血管外科研究室,安徽,合肥,230011 2. 中国科学院上海曼迪生物材料中试站,上海,200333 3. 上海第二医科大学第九人民医院血管外科研究室,上海,200011 |
| 摘 要： | 目的 :研究脱除犬血管细胞获取完整血管基质的方法。方法 :采用不同浓度的去垢剂和蛋白酶 ,以及不同温度和时间的效果比较 ,并通过组织学检查 ,确定犬血管脱细胞细胞外基质的较好方法。结果 :经低渗 /高渗溶液加去垢剂和蛋白酶抑制剂处理 ,犬血管的细胞全部脱除 ,细胞外基质保持完好。结论 :制备血管脱细胞细胞外基质时去垢剂脱细胞的效果最佳 |
| 关 键 词： | 血管 细胞外基质 狗 |
| 文章编号： | 1009-9905(2002)-01-0014-04 |
| 修稿时间： | 2001年10月8日 |
Experimental study on preparation of acellular vascular graft matrix |
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| LIU Xian luo,SUN Da kuan,QIAN Xiao xing,LIU Xiao gang,CHEN Ke hui,HU Kai bing Research.Experimental study on preparation of acellular vascular graft matrix[J].Chinese Journal of Current Advances in General Surgery,2002,5(1):14-17. | |
| Authors: | LIU Xian luo SUN Da kuan QIAN Xiao xing LIU Xiao gang CHEN Ke hui HU Kai bing Research |
| Institution: | LIU Xian luo,SUN Da kuan,QIAN Xiao xing,LIU Xiao gang,CHEN Ke hui,HU Kai bing Research Department of Vascular Surgery,Hefei Municipal Second People's Hospital,Anhui Province |
| Abstract: | Objective:To access the preparation of acellular vascular matrix in dogs.Methods:The best method of preparation of acellular vascular matrix in dogs was confirmed by comparison of the effectiveness of solutions of different detergents and proteinase with different temperature and period of time,and histological examination.Results:A multistep process involving hypotonic and hypertonic solutions,detergents and proteinase inhibitor may remove all cells with the extracellular matrix well conserved.Conclusion:The acellular vascular graft matrix is well prepared with detergents. |
| Keywords: | Blood vessels Extracellular matrix Dogs |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |