p53功能在肝癌中表达的意义

为探讨Ｐ５３功能及某些生物学行为肝癌中表达的意义。方法：收集１９９７年１月至１９９８年１２月５２例原发性肝癌的临床资料，并对这５２例手术切除的肝癌标本，分别用Ｗｅｓｔｅｒｎ ｂｌｏｔ结合ＤＮＡ损伤诱导的方法检测野生型Ｐ５３的功能，免疫组化检测Ｐ５３蛋白的表达，ＰＣＲ／ＳＳＣＰ方法检测Ｐ５３基因在第５－８外显子的丢失情况及免疫组化检测肿瘤增殖细胞核抗原（ＰＣＮＡ）指数。结果：５２例标本中，３１例（５

Value of p53 functional expression in hepatocellular carcinoma

Objective: To observe the p53 function, some of pathological and molecular behaviors of hepatocellular carcinoma (HCC). Methods: The function of wild-type P53 was tested using Western blot analysis and DNA damge agent in ducing test for 52 specimens of HCC from January 1997 to December 1998. These 52 specimens were also examined immunohistochemically us ing anti -p5 3 and anti- PCNA monoclonal antibodies. For mutations of p53 alterations in exons 5 ~8 of the gene were determined using the single-stranded conformational polymorphism analysis of polymerase chain reaction products (PCR/SSCP) method. Results: Loss of wild-type p53 function was found in 31 cases (59. 6% ) of these 52 specimens. There were 31 cases (59. 6% ) with p53 protein positive expression and 27 cases (51. 9% ) with p53 mutation. p53 function had parallel correlation to p53 protein expression (r = 0. 6006, P =0. 00001) and to p53 gene mutation (r = o. 5879, P = o. o()Ool ). PCNA high index appeared in 3o cases (57. 7% ). ln the 3l sPecimens which loss of p53 function, 26 cases showed also p53 protein positive expression(83. 9% ), p53 gene mutation in 24 cases (77. 4% ),PCNA high index in 24 cases (77. 4% ). This data showed that p53 function had close relationship with the liver cirrhosis.Loss wild-type p53 function usually appeared in the HCC with liver cirrhosis (P = 0. 0015 ). Conclusion: High index of PCNA, positive expression of p53 protein, p53 gene mutation frequently appeared in the cases with loss of wild-type p53 function (all of the P < 0. 01 ). Also when the tumor appeared loss of wild- type p53 function, the tumor showed early intrahepatic metastasis, porial vein embolus and growth more rapidly.