阿德福韦酯对慢性乙型肝炎患者外周血树突状细胞和淋巴细胞亚群的影响

目的 观察慢性乙型肝炎（CHB）患者阿德福韦酯（ADV）抗病毒治疗前后外周血树突状细胞（DC）和淋巴细胞亚群动态变化，探讨ADV抗HBV的免疫效应机制。方法 对15例ADV抗病毒治疗有效的CHB患者动态观察1年，设健康对照组10例。在治疗前、治疗3个月、6个月和1年对外周血单核细胞源DC进行体外培养，流式细胞仪检测分析DC表面分子及淋巴细胞亚群水平。结果 15例CHB患者均治疗持续有效。与治疗前比较，治疗3个月时DC表面分子CD1a、CD83、CD86和MHC-DR的表达无明显变化；治疗6个月和1年时上调，但二时相比较差异无显著性，且均低于健康对照组。淋巴细胞亚群于抗病毒3个月时亦无明显变化；治疗6个月和1年时，CD4+T及CD4+/CD8+T比值显著提高；NK比例虽有上升趋势，但与治疗前比较无统计学意义；CD3+T、CD8+T和CD19+B细胞未见明显改变。结论 ADV治疗后CHB患者免疫增强，提示其可能通过调节DC和淋巴细胞亚群的功能而参与免疫应答，发挥间接抗病毒作用。

Effects of adefovir dipivoxil on peripheral blood dendritic cells and lymphocyte subsets in patients with chronic hepatitis B

Objective To observe the dynamic changes of dendritic cells(DCs) and lymphocytes subsets from peripheral blood of patients with chronic hepatitis B(CHB) treated with adefovir dipivoxil(ADV) and explore the influence on the cellular immunity after ADV therapy.Methods Fifteen CHB patients treated with ADV and ten health controls were included and followed up for one year in this study.DCs were isolated from peripheral blood and cultured in vitro at baseline,3,6 and 12 months.Subsequently,flow cytometry(FCM) w...