骨髓间充质干细胞条件培养基对缺氧大鼠神经元凋亡的影响

目的观察大鼠骨髓间充质于细胞（BMMSCs）条件培养基（B—CM））对缺氧大鼠大脑皮质神经元凋亡的影响。方法分离、培养、传代Wistar大鼠BMMSCs，细胞融合达90％时更换培养基，继续培养24h后收集培养上清液即B—CM；取培养8d的新生Wistar大鼠大脑皮质神经元，分正常对照组、缺氧组、B—CM组，分别于缺氧0、6h、复氧24h用四甲基偶氮唑盐微量反应比色（MTT）法检测各组细胞活性，用流式细胞仪和透射电镜技术检测神经元凋亡情况。结果神经元缺氧6h细胞活性较对照组明显降低，复氧24h后细胞凋亡率明显升高（P〈0．01）。B—CM培养后细胞活性和缺氧前无差异，细胞凋亡明显较少（P〈0．01）。结论B—CM对缺氧一复氧引起的神经元凋亡有明显的防护作用。

Effects of Bone Marrow Mescenchymal Stem Cells Conditioned-Medium on Cortical Neuronal Apoptosis Induced by Hypoxia-Reoxygenation in vitro in Rats

Objective To observe the effects of bone marrow mesenchymal stem cells(BMMSCs) conditioned-medium (B-CM) on apoptosis in cultured cortical neurons after hypoxia-reoxygenation in vitro in rats.Methods BMMSCs were isolated,cultured and expanded,the culture me-dium was substituted by new medium that contain no serum when cells grew to 90% confluence.After cultured for 24 hours,the medium was collected as B-CM.Cortical neurons of mouse cultured for 8 days were divided into three groups:normal control groups,hypoxic group,B-CM disposed groups.Cell viability were detected by 3-(4,5-dimethlthiazd-2-yl)-2,5-diphenyltetra-zoliu bromidel (MTT) methods and the apoptosis of neurons was examined by flow cytometry and telemicroscopy on hypoxia for 0,6 hours and hypoxia-reoxygenation for 24 hours,respectively.Results Compared with control group,hypoxia for 6 hours could decrease cell viability,hypoxia-reoxygenation for 24 hours increased the cell apoptosis ratio remarkably(P<0.01),and B-CM could ameliorate cell apoptosis(P<0.01),but the cell viability were not af-fected.Conclusion B-CM can ameliorate neuronal apoptosis induced by hypoxia-reoxygenation.