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Identification and characterisation of ten microsatellite loci in the Noisy Scrub-bird Atrichornis clamosus using next-generation sequencing technology |
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| Authors: | S J Cowen R J N Allcock S J Comer J D Wetherall D M Groth |
| Institution: | 1. Curtin Health Innovation Research Institute, School of Biomedical Sciences, Curtin University, GPO Box U1987, Perth, WA, 6845, Australia 4. Department of Environment and Conservation, 120 Albany Highway, Albany, WA, 6330, Australia 2. LotteryWest State Biomedical Facility—Genomics, School of Pathology and Laboratory Medicine, University of Western Australia, Stirling Highway, Nedlands, Perth, WA, 6000, Australia 3. Department of Clinical Immunology and Immunogenetics, Royal Perth Hospital, Perth, WA, 6847, Australia |
| Abstract: | The Noisy Scrub-bird is an endangered species of songbird endemic to the south coast of Western Australia that has undergone a major and prolonged population bottleneck. Using shotgun 454 next-generation DNA sequencing we have identified and characterised ten polymorphic microsatellite loci in this species. Observed allelic diversity was relatively low (2–5 alleles per locus) and significant deviations from Hardy–weinberg Equilibrium observed, although the presence of null alleles was only postulated for two loci. The microsatellite loci characterised in this study will be useful in a future population genetics studies in this endangered species. |
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