| 甲状腺球蛋白抗原决定簇基因的克隆及其真核表达载体的构建 |
| |
| 引用本文： | 冯洁,李宁,吕枚,方佩华,杨立平,崔景秋,王少艳,张红艳. 甲状腺球蛋白抗原决定簇基因的克隆及其真核表达载体的构建[J]. 武警医学院学报, 2006, 15(1): 10-13 |
| |
| 作者姓名： | 冯洁 李宁 吕枚 方佩华 杨立平 崔景秋 王少艳 张红艳 |
| |
| 作者单位： | 天津医科大学总医院核医学科,天津,300052 |
| |
| 摘 要： | [目的]构建可将甲状腺球蛋白抗原决定簇基因转染入真核细胞的重组载体。[方法]用TRIzol一步法从甲状腺组织中提取总RNA,RT-PCR逆转录出cDNA,PCR扩增出目的基因,与pcDNATM3．1D／V5-His- TOPO(?)载体进行拓扑克隆,对重组质粒进行PCR、酶切及测序鉴定。[结果]甲状腺球蛋白抗原决定簇基因及其重组载体经鉴定准确无误。[结论]成功的构建了带有目的基因的真核表达质粒Tg-pcDNATM3．1D／V5-His- TOPO(?)。
|
| 关 键 词： | 甲状腺球蛋白 抗原决定簇基因 真核表达载体 |
| 文章编号： | 1008-5041（2006）01-0010-04 |
| 收稿时间： | 2005-09-03 |
| 修稿时间： | 2005-11-10 |
Cloning of thyroglobulin epitopes gene and construction of its eukaryotic expression vector |
| |
| FENG Jie,LI Ning,LU Mei,FANG Pei-hua,YANG Li-ping,CUI Jing-qiu,WANG Shao-yan,ZHANG Hong-yan. Cloning of thyroglobulin epitopes gene and construction of its eukaryotic expression vector[J]. Acta Academiae Medicinae CPAPF, 2006, 15(1): 10-13 |
| |
| Authors: | FENG Jie LI Ning LU Mei FANG Pei-hua YANG Li-ping CUI Jing-qiu WANG Shao-yan ZHANG Hong-yan |
| |
| Abstract: | [Objective] The recombined vector that can transfer thyroglobulin(Tg) epitopes gene into cells was constructed. [Methods] Total RNAs were extracted from human thyroid tissues of patient with Graves' disease (GD) by TRIzol reagent. The Tg epitopes gene was amplified by RT-PCR. The target gene was inserted into the expressive vectors of pcDNA?3. 1D/VS-His-TOPO(?) and then identified by PCR, restriction endonuclease and sequencing. [Results] The recombined vector with Tg epitopes gene was correct by identifying. [Conclusion] The eukaryotic expression vector Tg-pcDNA?.1D/V5-His-TOPO(?) with objective gene was constructed successfully. |
| |
| Keywords: | Thyroglobulin Epitopes Eukaryotic expression vector |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
