中药复方二神丸主要化学成分的高效液相-飞行时间质谱分析

目的通过高效液相-飞行时间质谱（HPLC—TOF／MS）联用技术，对中药复方二神丸中的主要化学成分进行有效的鉴定。方法色谱分离采用资生堂Capcell Pak C18反相柱（3．0mm×100nmm，3μm），流动相组成分别为0．1％甲酸水溶液和乙睛，梯度洗脱，流速为0．6ml／min；质谱定性采用飞行时间质谱，正离子模式扫描。结果在优化的液质联用条件下，通过飞行时间质谱鉴定出二神丸中21个成分，其中15种成分来自补骨脂，6种成分来自肉豆蔻。结论通过RRLC—TOF／MS联用技术，为鉴定二神丸中的化学成分建立起了一种快速、高效的分析方法。

Analysis on chemical constituents of ershen pill by HPLC-TOF/MS

Objective To analyze chemical constituents of ershen pill by rapid-resolution（ high performance） liquid chroma- tography-time of flight mass spectrometry （HPLC-TOF/MS）. Methods A shelloseido eoluinn（3.0 mm × 100 mm,3μm） was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.6 ml/min. TOF/MS was applied for qualitative analysis under positive ion mode. Results Under LC/MS condition, 21 major constituents in ershen pill were identified by time of flight mass spectrometry, among which, 15 constituents were from Psoralea corylifolia L,and 6 constituents were from Myristicafragrans Houtt. Conclusion A simple and reliable method using HPLC-TOF/MS was established to identify the chemical constituents of ershen pill.