趋化因子受体CCR5在胃癌患者外周血调节性T细胞上的表达及其临床意义

目的检测胃癌患者外周血中CD4+CD25+CD127-调节性T细胞(Tregs细胞)上的趋化因子受体5(CCR5)的表达水平,并探讨其与胃癌发生、侵袭和转移的相关性。方法利用流式细胞仪检测福建医科大学附属泉州第一医院2013年1月至2014年2月49例胃癌患者与20例健康对照组的外周血Tregs细胞上CCR5的表达水平并进行统计分析。结果胃癌患者组外周血Tregs细胞上CCR5表达水平明显高于健康对照组(P0.05);CCR5在胃癌外周血Tregs细胞上的表达水平与患者年龄、肿瘤直径无关(P0.05),但与组织学分级、病理分期及淋巴结转移相关(P0.05)。多元线性回归分析结果显示:CCR5在胃癌患者外周血Tregs细胞表达水平与性别、年龄、分化程度、肿瘤大小无统计学意义的线性关系,而与病理分期及淋巴结转移呈线性相关。秩相关分析显示:CCR5在胃癌患者外周血Tregs细胞表达水平与性别、年龄、肿瘤大小无统计学意义的相关关系,与病理分期及淋巴结转移呈正线性相关。结论 CCR5在胃癌患者外周血Tregs细胞表达上调,明显高于健康对照组,并与胃癌的病理分期和淋巴结转移关系密切,提示病理分期越高或者淋巴结转移程度越广泛,CCR5在胃癌患者外周血Tregs细胞上表达就越高。CCR5与外周血Tregs细胞的相互介导可能参与机体的肿瘤免疫,在胃癌的发生、发展及侵袭转移中可能起重要作用。

Expression of chemokine receptor 5 in peripheral blood regulatory T cells on gastric cancer patients and its clinical significance

Objective To investigate the expression and significance of chemokine receptor 5 in peripheral blood CD4 ＋ CD25 ＋ CD127 - regulatory T cells on patients with gastric cancer and their relevant healthy controls. To explore its relationship with the occurrence, invasion and metastasis of gastric cancer. Methods Using flow cytometry technique（FCM） detection of the expression level of CCR5 in CD4 ＋ CD25 ＋ CD127 - regulatory T cells on 49 gastric cancer patients and 20 healthy controls peripheral blood and to study relationship of the expression level with clinical pathological parameters. SPSS19.0 was used for stastistical analyzing. T test and One-way ANOVA were used for measurement data. Linear regressssion and spearman test were used for association between groups. Results （ 1 ） The expression level of CCR5 in peripheral blood CD4 ＋ CD25 ＋ CD127 - regulatory T cells on gastric cancer patients were evidently higher than that under healthy controls, and the difference was significant; （2） The expression level of CCR5 in peripheral blood CD4 ＋ CD25 ＋ CD127 -regulatory T cells was not related with the age of patient, gender difference, tumor size, but was relevant to the histologic grade, clinical stage of the cancer and lymph node metastasis; （3） The expression level of CCR5 in peripheral blood CD4 ＋ CD25 ＋ CD127 - regulatory T cells was positively linear correlated with lymph node metastasis or pathological stage on gastric cancer, but not with the age of patient, gender difference, tumor size and histologic grade; （4） The expression level of CCR5 in peripheral blood CD4 ＋ CD25 ＋ CD127 - regulatory T cells was not correlated with the age of patient, gender difference, tumor size, but with pathological stage or lymph node metastasis. Conclusions The expression of CCR5 in CD4 ＋ CD25 ＋ CD127 - regulatory T cells may be relevant to tumor immunity, and may play an important role in the occurrence, invasion and metastasis of gastric cancer.