新型介孔二氧化硅纳米微球/羟基磷灰石/生物玻璃复合生物涂层的体外理化特性

目的本实验设计制备了介孔硅纳米微球(mesoporous silica nanoparticulate,MSN)/羟基磷灰石(hydroxyapatite,HA)/生物玻璃(bioactive glass,BG)复合生物涂层。并对其加载唑来膦酸(Zoledronic acid,ZOL)后的体外药物释放特性进行了研究。方法通过扫描电镜、透射电镜及能谱仪等方法观察H/M涂层表征。通过高效液相色谱法进行HA/MSN/BG、HA/MSN及HA生物涂层体外ZOL加载及释放的比较。结果通过扫描电镜观察HA/MSN涂层,发现其具有二氧化硅微球组成的多孔结构。体外药物实验中H/M比HA载药量大更且与初始药物浓度相关,并具有药物缓释的特性,涂布BG后缓释效应更加明显。结论 H/M/B因其具有载药量大及药物缓释特性,为生物涂层内固定物在骨折愈合上的应用提供了新技术。

In vitro characterizations of novel mesoporous silica nanoparticles/ hydroxyapatite /bioactive glass composite coat

Abstract] Objective The use biological coat in inner fixation is a new star of healing fracture and bone nonunion. In an attempt to improve implant-bone integration and accelerate bone fracture healing, we prepared a novel mesoporous silica nanoparticulate/ hydroxyapatite/bioactive glass composite coat. Characterizations and drug release characters which was loaded with Zoledronic acid was investigated in our experiment. Methods Characterizations of H/M coating was investigated by SEM, TEM and EDS. The H/M/B composite coat was contrasted with HA coat in the drug load and release experiment by HPLC. Result Pictures of SEM and TEM show that innumerable micropores was formed by silica nanoparticulates on the H/M coat. Bigger drug load and sustained release of drug was observed compared to HA coat. Sustained releasing was more obvious when H/M was coated by bioactive glass. Conclusion The novel composite coat had a series merits compared to actual biological coat like big load of drug, sustained drug release, economic and non-toxic. It gave us a new choice of inner fixation to deal with fracture in the future.