高血脂兔主动脉粘附分子VCAM-1基因表达的研究

目的：研究高血脂对主动脉血管细胞粘附分子１（ＶＣＡＭ－１）基因表达的影响。方法：采用胆固醇喂饲法建立兔高血脂模型,在喂饲后１,６周处死动物,应用组织原位杂交、免疫组织化学等方法检测产动脉ＶＣＡＭ－１的表达,并与正常组对比。结果：喂饲胆固醇１周后高血脂很快诱导主动脉内皮细胞表达ＶＣＡＭ－１,并在６周后动脉粥样硬化（ＡＳ）病灶内也有丰富表达。结论：高血脂促进动脉ＶＣＡＭ－１表达,是其促进ＡＳ发病的机制

A study on gene expression of VCAM-1 in aortas of hypercholesterolemic rabbits

Objective: Circulating monocyte binding to vascular endothelial cell is an important step in atherosclerosis. Vascular cell adhesion molecule 1(VCAM 1) has a pivotal role in mediating adhesion of these two kinds of cells. The current study was designed to evaluate the effect of hypercholesterolemia on inducing gene expression of VCAM 1 in aortas of rabbits. Methods: Rabbits were fed with cholesterol to create hypercholesterolemic animal model. At the end of 1st week and 6th week, the animal were killed. Using in situ hybridization and immunohistochemistry, we check the expression of VCAM 1 in aorta. Results: we found that hypercholesterolemia quickly induced gene expression of VCAM 1 in the aortic endothelial cells after 1 week of cholestrol feeding, and there was also a high expression in the atherosclerosis lesion 5 weeks later. Conclusion: These results indicate that inducing gene expression of VCAM 1 is one of atherogenic mechanisms of hypercholesterolemia.