黄芪多糖对2型糖尿病ZDF大鼠血糖及肝脏脂代谢影响

目的观察黄芪多糖对2型糖尿病ZDF大鼠血糖及肝脏脂代谢的影响。方法选取30只成年雄性ZDF大鼠,随机分为模型组、APS低剂量、APS中剂量组、APS高剂量组和二甲双胍组;另取6只成年雄性ZDF(fa/+)大鼠作为正常组。实验组灌胃给予相应剂量药物,模型组和正常组灌胃给予生理盐水溶液,测定各组ZDF大鼠体重、空腹血糖;肝脂代谢指标:TC、TG、LDL-C、HDL-C含量;肝损伤指标:AST、ALT活性;肝脏病理学检测,从而探讨APS对2型糖尿病ZDF大鼠血糖及肝脏脂代谢的影响。结果给药前实验组大鼠体重、空腹血糖显著增高,TC、TG、LDL-C显著增加,符合糖尿病症状,且肝损伤存在一定程度损伤;予APS后各实验组大鼠体重较模型组显著降低(P0.05),同时与给药前相比体重显著性降低(P0.05);空腹血糖较模型组显著性降低(P0.05),其中黄芪多糖大剂量降血糖作用最为显著(P0.01),与给药前相比得出同样的结论;各实验组大鼠TC、TG、LDL-C较模型组均有降低趋势,其中APS高剂量组TC、TG、LDL-C含量下降最显著(P0.05);肝损伤方面,各给药组大鼠ALT、AST较模型组均有降低趋势,且APS高剂量组最为显著(P0.05);肝脏HE结果显示模型组细胞核皱缩,胞质呈网丝状,细胞间存在明显脂滴沉积;予APS后,肝脏细胞形态趋于正常,部分细胞内存在细小脂滴,肝小叶结构正常,肝细胞索呈放射状排列。结论黄芪多糖通过降血糖和纠正肝脏脂代谢紊乱,改善肝脏脂滴沉积,对抗糖尿病引起的肝损伤,从而起到抗2型糖尿病的作用。

Effects of astragalus polysaccharides on blood glucose and liver lipid Metabolism in ZDF Rats with type 2 diabetes mellitus

Objective To observe the effect of astragalus polysaccharides on blood glucose and liver lipid metabolism in ZDF rats with type 2 diabetes mellitus.Methods 30 adult male ZDF rats were selected and randomly divided into model group,APS low dose group,APS middle dose group,APS high dose group and metformin group.6 adult male ZDF(fa/+)rats were selected as normal group.The rats in the experimental group were treated with corresponding dose of the drug.The model group and normal group were given the same dose of saline solution.The body weight,fasting blood glucose,liver lipid metabolism indexes(TC,TG,LDL-C and HDL-C),liver damage indexes(AST,ALT activity)and liver pathology detection of ZDF rats in each group were determined,explored the influence of APS on blood glucose and liver lipid metabolism in type 2 diabetes ZDF rats.Results The body weight,fasting blood glucose,TC,TG and LDL-C were significantly increased in the experimental group before drug administration,which was accorded with the symptoms of diabetes mellitus and there was a certain degree of damage to liver.After administration of APS,the body weight of each experimental group was significantly lower than that of the model group and that before drug treatment(P<0.05).Fasting blood glucose in each treatment group was significantly lower than that in model group,and the high dose of astragalus polysaccharides had the most significant hypoglycemic effect,which got the same conclusion to that before administration.The levels of TC,TG and LDL-C in each experimental group were lower thanthose in the model group,and the decrease of TC,TG,LDL-C in APS high dose group was the most significant(P<0.05).The ALT and AST in each treatment group were lower than those in the model group,and the decrease in APS high dose group was the most significant(P<0.05).Liver HE results showed that there was shrinkage of nucleus in the model group,and the cytoplasm was filamentous,and there was significant lipid deposition between cells.After the administration of APS,the liver cell morphology tended to normal,and there were small lipid droplets in some cells,with normal liver lobular structure and radially arranged in hepatic cords.Conclusion Astragalus polysaccharides can improve liver lipid deposition and prevent hepatic injury caused by diabetes mellitus by lowering blood sugar and correcting liver lipid metabolism disorder,so as to play an role of anti-type 2 diabetes mellitus.