Gene identification by chromosomal in-situ hybridization, microdissection and polymerase chain reaction amplification |
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Abstract: | This paper describes a theoretical method by which candidate genes for inherited diseases can be identified by polymerase chain reaction amplification and subsequent cloning of organ-specific complementary deoxyribonucleic acid libraries hybridized in situ to, and subsequently dissected from, the cognate chromosome. A specific application of this technique to isolate genes related to genetic haemochromatosis is outlined. |
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