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大气生物气溶胶实时监测与Andersen6级采样结果的对比研究
引用本文:李娜,鹿建春,温占波,王洁,杨文慧,李劲松. 大气生物气溶胶实时监测与Andersen6级采样结果的对比研究[J]. 军事医学科学院院刊, 2011, 35(7): 527-530
作者姓名:李娜  鹿建春  温占波  王洁  杨文慧  李劲松
作者单位:军事医科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,国家生物防护装备工程技术研究中心,北京100071
基金项目:国家自然科学基金资助项目
摘    要:目的研究利用荧光分析识别生物粒子的实时监测技术和Andersen6级采样器采样对大气生物气溶胶采样结果的影响,为大气生物气溶胶监测方法提供理论依据。方法利用生物粒子实时监测采样仪采集大气生物气溶胶粒子,用Andersen6级采样器作为对照,比较两种方法采集的生物气溶胶粒子的浓度变化。结果实时监测采集到的生物粒子浓度为20~120个粒子(pts)/L,Andersen6级采样器检测到的微生物气溶胶菌落浓度集中在0.2~5cfu/L。实时监测每6s检测空气中生物粒子数,可实时、直观反映空气中粒子浓度的变化,而Andersen6级采样器检测结果是一定采样时间内的生物气溶胶的菌落浓度,需要较长的培养时间。两种采样方法检测到的大气生物气溶胶粒子浓度总的年度变化趋势基本一致,但是浓度值在不同采样时段分散性很大。结论实时监测采样灵敏度高,可及时反映生物粒子浓度的变化情况,比传统采样方法更适用于实时监测大气生物气溶胶本底变化规律,帮助判断是否存在生物气溶胶暴露的危害。

关 键 词:大气生物气溶胶  实时监测  Andersen6级采样器

Real-time ambient bioaerosols monitoring and the Andersen six-stage sampling result:a comparative study
LI Na,LU Jian-chun,WEN Zhan-bo,WANG Jie,YANG Wen-hui,LI Jin-song. Real-time ambient bioaerosols monitoring and the Andersen six-stage sampling result:a comparative study[J]. Bulletin of the Academy of Military Medical Sciences, 2011, 35(7): 527-530
Authors:LI Na  LU Jian-chun  WEN Zhan-bo  WANG Jie  YANG Wen-hui  LI Jin-song
Affiliation:LI Na,LU Jian-chun,WEN Zhan-bo,WANG Jie,YANG Wen-hui,LI Jin-song(State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing 100071,China)
Abstract:Objective To provide a theoretic basis for ambient bioaerosols monitoring by comparing the sampling results by real-time monitoring with fluorescence analysis technique and Andersen six-stage sampler.Methods The bioaerosols in the ambient air were collected by a biological particle real-time monitor,and simultaneously an Andersen six-stage sampler was used as a comparison.The concentration changes of bioaerosols were refleced by the results of the two sampling methods.Results The concentration of bioaerosol...
Keywords:Andersen
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