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| 肝硬化患者血浆中AFP、PKM2和ALB mRNA的检测与分析 | |
| 引用本文： | 曲秋月,于源滋,李杰,付汉江,铁轶,郭明洲,郑晓飞.肝硬化患者血浆中AFP、PKM2和ALB mRNA的检测与分析[J].军事医学科学院院刊,2011,35(7):500-504. |
| 作者姓名： | 曲秋月 于源滋 李杰 付汉江 铁轶 郭明洲 郑晓飞 |
| 作者单位： | 1. 军事医学科学院放射与辐射医学研究所,北京,100850 2. 解放军总医院消化科,北京,100853 3. 军事医学科学院放射与辐射医学研究所,北京100850;安徽医科大学生化与分子生物学教研室,合肥,230032 |
| 基金项目： | 艾滋病和病毒性肝炎等重大传染病防治国家重大专项资助项目，北京市自然科学基金资助项目 |
| 摘 要： | 目的探讨乙肝肝硬化患者外周血血浆中甲胎蛋白（AFP）、丙酮酸激酶M2（PKM2）和白蛋白（ALB）mRNA的表达水平及其临床意义。方法采用实时定量PCR方法检测了38例健康人血浆及38例乙肝肝硬化患者血浆中AFP mRNA、PKM2 mRNA和ALB mRNA的表达水平,并分析其表达与临床检测指标间的相关性。结果 AFPmRNA、PKM2 mRNA及ALB mRNA的表达在肝硬化患者血浆和健康人血浆中存在明显差异,结果具有统计学意义（P〈0.01）。AFP mRNA和PKM2 mRNA的表达与临床检测指标没有显著相关性,ALB mRNA与γ-谷氨酰基转移酶及血清白蛋白具有相关性。与甲胎蛋白相比,检测的3种血浆mRNA在乙肝肝硬化患者中的高表达更明显。结论检测外周血血浆中AFP mRNA、PKM2 mRNA和ALB mRNA的表达对肝病的临床诊断及治疗具有指导意义。 |
| 关 键 词： | mRNA 乙肝肝硬化 血浆 实时定量PCR 甲胎蛋白类 白蛋白类 |
Detection and analysis of mRNA of alfa-fetoprotein,pyruvate kinase type M2 and albumin in plasma of cirrhosis patients |
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| QU Qiu-yue,YU Yuan-zi,LI Jie,FU Han-jiang,TIE Yi,GUO Ming-zhou,ZHENG Xiao-fei.Detection and analysis of mRNA of alfa-fetoprotein,pyruvate kinase type M2 and albumin in plasma of cirrhosis patients[J].Bulletin of the Academy of Military Medical Sciences,2011,35(7):500-504. | |
| Authors: | QU Qiu-yue YU Yuan-zi LI Jie FU Han-jiang TIE Yi GUO Ming-zhou ZHENG Xiao-fei |
| Institution: | QU Qiu-yue1,YU Yuan-zi2,LI Jie1,3,FU Han-jiang1,TIE Yi1,GUO Ming-zhou2,ZHENG Xiao-fei1(1.Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China,2.Department of Gastroenterology and Hepatology,PLA General Hospital,Beijing 100853,3.Anhui Medical University,Hefei 230032,China) |
| Abstract: | Objective To investigate the expression level and clinical significance of mRNA of alpha-fetoprotein(AFP),pyruvate kinase type M2(PKM2) and albumin(ALB) in the plasma of cirrhosis patients with hepatitis B virus(HBV).Methods Real-time quantitative PCR was used to detect the expression level of mRNA of AFP,PKM2 and ALB in the plasma of cirrhosis patients with HBV,and the correlation between the expression level and the clinical biochemical indexes was analyzed.Results The expression level of mRNA of AFP,PKM2... |
| Keywords: | mRNA |
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