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| N-乙酰半胱氨酸对软脂酸培养中胰岛细胞的影响 | |
| 引用本文： | 王露,张微,彭观景,赵晟.N-乙酰半胱氨酸对软脂酸培养中胰岛细胞的影响[J].陕西医学杂志,2011,40(7):785-786. |
| 作者姓名： | 王露 张微 彭观景 赵晟 |
| 作者单位： | 1. 中国医科大学附属第四医院内分泌科,沈阳,110032 2. 中国医科大学附属盛京医院第一微创胆道血管外科 3. 中国医科大学附属盛京医院内分泌科 |
| 摘 要： | 目的:探讨N-乙酰半胱氨酸(NAC)对软脂酸培养下胰岛细胞的影响。方法:体外培养胰岛细胞INS-1,分为空白对照组,软脂酸组(0.25 mmol/L),NAC作用组(浓度分别为50μmol/L,100μmol/L,200μmol/L),培养24h后,取上清测定胰岛素水平及GSH-Px、MDA含量。结果:软脂酸可使胰岛素分泌量减少,GSH-Px浓度下降,MDA含量增加,NAC可以明显抑制软脂酸引起的上述变化,抑制作用随NAC浓度的增加而增强。结论:抗氧化剂NAC对软脂酸导致的胰岛细胞损伤有一定的保护作用,并且呈浓度依赖性。 |
| 关 键 词： | 乙酰半胱氨酸 脂肪酸类 谷胱甘肽过氧化酶/分析 丙二醛/分析 胰岛素/分析 |
Effect of N-acetylcysteine on islet cell cutueled with palmitic acid |
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| Institution: | Department of Endocrinology,The Fourth Affiliated Hospital of ChinaMedical University(Shenyang 110032) Wang Lu Zhang Wei Peng Guanjing et al |
| Abstract: | Objective：To investigate the effect of N-acetylcysteine on islet cell cultrued with palmitic acid.Methods：The cultrued islet cells（INS-1）were divided into 5 groups：normal control group,palmitic acid（PA） group（0.25mmol/L）,50μmol/L NAC＋ PA group,100μmol/L NAC＋ PA group,200μmol/L NAC＋ PA group.The level of insulin,GSH-Px and MDA in Culture supernatant were measured when cultrued for 24h.Result：Palmitic acid had the effects of inhibiting insulin secretion of pancreatic islet cell,reducing the concentration of GSH-Px and enhancing the level of MDA.This change were inhibited by NAC with a dose-dependent manner.Conclusion：NAC protected islet cell against injury induced by palmitic acid through inhibiting oxidation in dose-dependent manner. |
| Keywords: | Acetylcysteine Fatty acids Glutathione peroxidase/analysis Malondialdehyde/analysis Insulin/analysis |
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